一氧化氮在人离体结肠抑制性神经支配中的作用
Involvement of nitric oxide in the inhibitory innervation of the human isolated colon.
作者信息
Boeckxstaens G E, Pelckmans P A, Herman A G, Van Maercke Y M
机构信息
Division of Gastroenterology, Faculty of Medicine, University of Antwerp, Antwerpen-Wilrijk, Belgium.
出版信息
Gastroenterology. 1993 Mar;104(3):690-7. doi: 10.1016/0016-5085(93)91003-z.
BACKGROUND
The exact nature of the inhibitory nonadrenergic noncholinergic (NANC) neurotransmitter in the human colon is still unknown.
METHODS
The present study was designed to investigate the role of nitric oxide (NO) and adenosine 5'-triphosphate (ATP) in circular muscle strips of the human isolated colon.
RESULTS
NO and ATP induced tetrodoloxin-resistant relaxations that mimicked those evoked by nerve stimulation. Apamin inhibited the response to ATP, had a variable effect on the relaxations to transmural stimulation, and had no effect on those to NO or nitroglycerin. NG-nitro-L-arginine (L-NNA) concentration dependently reduced the NANC nerve-mediated relaxations, but had no effect on those to ATP, NO, or nitroglycerin; the L-NNA resistant part of the NANC relaxation to nerve stimulation was further reduced by apamin. The inhibitory effect of L-NNA or the combination of L-NNA and apamin was prevented by L-arginine but not by D-arginine.
CONCLUSIONS
These results suggest that NO and another substance, perhaps ATP, are involved in the inhibitory NANC neurotransmission in the circular muscle of the human colon.
背景
人类结肠中抑制性非肾上腺素能非胆碱能（NANC）神经递质的确切性质仍不清楚。
方法
本研究旨在探讨一氧化氮（NO）和5'-三磷酸腺苷（ATP）在人离体结肠环形肌条中的作用。
结果
NO和ATP诱导了对河豚毒素耐药的舒张，这种舒张模拟了神经刺激所引起的舒张。蜂毒明肽抑制了对ATP的反应，对跨壁刺激引起的舒张有不同影响，对NO或硝酸甘油引起的舒张无影响。NG-硝基-L-精氨酸（L-NNA）浓度依赖性地降低了NANC神经介导的舒张，但对ATP、NO或硝酸甘油引起的舒张无影响；蜂毒明肽进一步降低了NANC对神经刺激舒张中L-NNA耐药的部分。L-精氨酸可防止L-NNA或L-NNA与蜂毒明肽联合使用的抑制作用，而D-精氨酸则不能。
结论
这些结果表明，NO和另一种物质（可能是ATP）参与了人类结肠环形肌中的抑制性NANC神经传递。
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