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生长温度对荧光假单胞菌脂质的影响。

Effect of growth temperature on the lipids of Pseudomonas fluorescens.

作者信息

Gill C O

出版信息

J Gen Microbiol. 1975 Aug;89(2):293-8. doi: 10.1099/00221287-89-2-293.

DOI:10.1099/00221287-89-2-293
PMID:809540
Abstract

Pseudomonas fluorescens was grown in co ntinuous culture with glucose or NH4+ as the growth-limiting substrate. The total amount of lipid and the relative proportions of neutral lipid and phospholipid did not vary with the rate or temperature of growth. The amounts of the phospholipids, which were phosphatidyl glycerol, cardiolipin and phosphatidyl ethanolamine, altered with the type of growth limitation and with the temperature and rate of growth. A precise composition of phospholipid classes is not a requirement for growth at low temperature. Fatty acid composition also varied with changes of these growth conditions at temperatures above 10 degrees C, but at lower growth temperatures the degree of saturation of the lipids was strictly controlled, and was unaffected by changes in the growth rate or the nature of the growth-limiting substrate. The degree of saturation of the lipids was not critical at the higher growth temperatures, and the variation in the degree of saturation observed at these temperatures was due in part to a decreased ability of the organism to control the fatty acid composition of its lipids at temperatures above 10 degrees C.

摘要

荧光假单胞菌在以葡萄糖或铵离子作为生长限制底物的连续培养中生长。脂质的总量以及中性脂质和磷脂的相对比例并不随生长速率或温度而变化。磷脂(磷脂酰甘油、心磷脂和磷脂酰乙醇胺)的含量随生长限制类型、温度和生长速率而改变。磷脂类的精确组成并非低温生长所必需。在高于10摄氏度的温度下,脂肪酸组成也随这些生长条件的变化而变化,但在较低的生长温度下,脂质的饱和度受到严格控制,且不受生长速率或生长限制底物性质变化的影响。在较高的生长温度下,脂质的饱和度并不关键,在这些温度下观察到的饱和度变化部分是由于该生物体在高于10摄氏度的温度下控制其脂质脂肪酸组成的能力下降。

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Effect of growth temperature on the lipids of Pseudomonas fluorescens.生长温度对荧光假单胞菌脂质的影响。
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