Merkel G J, Perry J J
Appl Environ Microbiol. 1977 Dec;34(6):626-9. doi: 10.1128/aem.34.6.626-629.1977.
The heat sensitivity of gram-negative, hydrocarbon-utilizing thermophilic bacteria was altered by a change in growth substrate. Thermophilic strains CC-6, BI-1, and LEH-1, grown with acetate or n-heptadecane as the carbon source, had a higher survival rate when incubated 5 degrees C above their maximum growth temperature than cells of the same organism after growth on glucose or glycerol. There was a correlation between the growth substrated, heat resistance, and the ratios of cellular n-hexadecanoic acid/branched hexadecanoic acid and n-heptadecanoic acid/branched heptadecanoic acid. The bacterial cells that were more heat resistant had ratios of straight-chain/branched-chain fatty acids above 1.0, whereas the heat-sensitive cells had ratios below 0.6.
利用碳氢化合物的嗜热革兰氏阴性菌的热敏感性会因生长底物的变化而改变。以乙酸盐或正十七烷作为碳源生长的嗜热菌株CC-6、BI-1和LEH-1，在高于其最高生长温度5摄氏度下培养时，比以葡萄糖或甘油生长后的同一种微生物细胞具有更高的存活率。生长底物、耐热性以及细胞中十六烷酸/支链十六烷酸和十七烷酸/支链十七烷酸的比例之间存在相关性。耐热性更强的细菌细胞直链/支链脂肪酸比例高于1.0，而热敏感细胞的比例则低于0.6。
