Palittapongarnpim P, Chomyc S, Fanning A, Kunimoto D
Department of Medical Microbiology and Infectious Diseases, Provincial Laboratory of Public Health for Northern Alberta, Edmonton, Canada.
J Infect Dis. 1993 Apr;167(4):975-8. doi: 10.1093/infdis/167.4.975.
Strain identification of Mycobacterium tuberculosis would prove whether transmission had occurred between individuals. A method to characterize strains of M. tuberculosis has been developed utilizing polymerase chain reaction (PCR). Purified chromosomal DNA of cultured clinical samples of M. tuberculosis were subjected to PCR using short (10-12 nucleotide) oligonucleotide primers. PCR products visualized after agarose gel electrophoresis and ethidium bromide staining demonstrated that different strains of M. tuberculosis give different banding patterns. This technique was used to confirm the relationship between cases of tuberculosis in several clusters, prove the lack of relationship between 2 isolates with the same antibiotic-resistance pattern, confirm a suspected mislabeling event, and suggest the source of infection in a case of tuberculous meningitis. This method is rapid and simple and does not require radioactive probes.
结核分枝杆菌的菌株鉴定将证明个体之间是否发生了传播。利用聚合酶链反应（PCR）开发了一种鉴定结核分枝杆菌菌株的方法。使用短（10 - 12个核苷酸）寡核苷酸引物对培养的结核分枝杆菌临床样本的纯化染色体DNA进行PCR。琼脂糖凝胶电泳和溴化乙锭染色后可视化的PCR产物表明，不同的结核分枝杆菌菌株呈现出不同的条带模式。该技术用于确认几个聚集性结核病病例之间的关系，证明具有相同耐药模式的2株分离菌之间没有关系，确认一次疑似标签错误事件，并提示一例结核性脑膜炎病例的感染源。该方法快速简便，且不需要放射性探针。
