用于结核分枝杆菌临床分离株分型的双重复元件PCR方法
Double-repetitive-element PCR method for subtyping Mycobacterium tuberculosis clinical isolates.
作者信息
Friedman C R, Stoeckle M Y, Johnson W D, Riley L W
机构信息
Division of Infectious Diseases, Cornell University Medical College, New York, New York 10021, USA.
出版信息
J Clin Microbiol. 1995 May;33(5):1383-4. doi: 10.1128/jcm.33.5.1383-1384.1995.
Abstract
We describe a rapid method for subtyping Mycobacterium tuberculosis based on PCR amplification of segments located between two distinct DNA repetitive elements. This method, double-repetitive-element PCR, classified 46 clinical isolates as having 25 distinct patterns; the conventional restriction fragment length polymorphism analysis classified the same isolates as having 23 distinct patterns. The double-repetitive-element PCR is a rapid subtyping method that has a discriminating power similar to that of the restriction fragment length polymorphism method.
摘要
我们描述了一种基于对位于两个不同DNA重复元件之间的片段进行PCR扩增来对结核分枝杆菌进行亚型分型的快速方法。这种方法，即双重复元件PCR，将46株临床分离株分为25种不同模式；传统的限制性片段长度多态性分析将同样的分离株分为23种不同模式。双重复元件PCR是一种快速亚型分型方法，其鉴别能力与限制性片段长度多态性方法相似。
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