Akatsuka M, Yamamoto Y, Tobetto K, Yasui T, Ando T
Division of Biochemical Pharmacology, Research Laboratories, Maruho Co. Ltd, Osaka, Japan.
J Pharm Pharmacol. 1993 Feb;45(2):110-4. doi: 10.1111/j.2042-7158.1993.tb03693.x.
Effects of hyaluronic acid on the release of elastase from rat peritoneal leucocytes were studied by measuring the leucocyte elastase activity using a synthetic peptide substrate. Leucocyte elastase release was induced by opsonized zymosan, 12-O-tetradecanoyl phorbol-13-acetate (TPA) and N-formyl-L-methionyl-L-leucyl-L-phenylalanine combined with cytochalasin B. Calcium ionophore A23187 potentiated the action of TPA on leucocyte elastase release, whereas hyaluronic acid inhibited leucocyte elastase release regardless of the method of stimulation. Inhibitory effects of hyaluronic acid were dependent on its concentration and molecular weight. Hyaluronic acid of the highest molecular weight (2.0 x 10(6)) indicated a potent inhibitory effect on elastase release. Our present findings suggest that hyaluronic acid may elicit an anti-inflammatory effect by inhibiting leucocyte elastase-dependent pathological processes.
通过使用合成肽底物测量白细胞弹性蛋白酶活性，研究了透明质酸对大鼠腹腔白细胞弹性蛋白酶释放的影响。经调理的酵母聚糖、12-O-十四烷酰佛波醇-13-乙酸酯（TPA）以及N-甲酰-L-蛋氨酰-L-亮氨酰-L-苯丙氨酸与细胞松弛素B联合诱导白细胞弹性蛋白酶释放。钙离子载体A23187增强了TPA对白细胞弹性蛋白酶释放的作用，而透明质酸无论采用何种刺激方法均抑制白细胞弹性蛋白酶释放。透明质酸的抑制作用取决于其浓度和分子量。最高分子量（2.0×10⁶）的透明质酸对弹性蛋白酶释放显示出强大的抑制作用。我们目前的研究结果表明，透明质酸可能通过抑制白细胞弹性蛋白酶依赖性病理过程发挥抗炎作用。
