Gadella B M, Colenbrander B, Van Golde L M, Lopes-Cardozo M
Department of Herd Health and Reproduction, Veterinary School, Utrecht University, The Netherlands.
Biol Reprod. 1993 Mar;48(3):483-9. doi: 10.1095/biolreprod48.3.483.
The presence and composition of arylsulfatases in secretions of various glands of the boar genital tract were studied. Arylsulfatase A was present in seminal plasma but not in extracellular fluids of the testis and epididymis nor in blood serum of boars. On the other hand, arylsulfatase B was present in both seminal plasma and extracellular fluids of the testis but was completely resorbed in the epididymis. The acrosomal arylsulfatase A did not leak out of spermatozoa before ejaculation. We conclude that arylsulfatases A and B present in seminal plasma are secreted by the seminal vesicles, for three reasons: 1) secretions from seminal vesicles contained 2.3-fold higher arylsulfatase activities than did those from seminal plasma, but had an identical composition; 2) cauda epididymal fluids did not contain arylsulfatase; and 3) other accessory glands of the boar genital tract did not secrete arylsulfatase. When intact boar spermatozoa were incubated with arylsulfatase A, complete desulfation of seminolipid was observed. The most important arguments favoring our hypothesis that desulfation of seminolipid does not start before ejaculation are the following: 1) desulfoseminolipid is not detectable in epididymal or freshly ejaculated sperm samples; 2) the acrosomal arylsulfatase A cannot desulfate seminolipid present at the surface of the plasma membrane of intact spermatozoa because of its intracellular localization; 3) extracellular arylsulfatase A is stored in seminal vesicles and thus can interact with spermatozoa during and after ejaculation.
研究了公猪生殖道各腺体分泌物中芳基硫酸酯酶的存在情况及其组成。芳基硫酸酯酶A存在于精浆中，但不存在于睾丸和附睾的细胞外液中，也不存在于公猪的血清中。另一方面，芳基硫酸酯酶B存在于精浆和睾丸的细胞外液中，但在附睾中被完全重吸收。顶体中的芳基硫酸酯酶A在射精前不会从精子中泄漏出来。我们得出结论，精浆中存在的芳基硫酸酯酶A和B是由精囊分泌的，原因有三点：1）精囊分泌物中的芳基硫酸酯酶活性比精浆中的高2.3倍，但组成相同；2）附睾尾液中不含芳基硫酸酯酶；3）公猪生殖道的其他附属腺体不分泌芳基硫酸酯酶。当完整的公猪精子与芳基硫酸酯酶A一起孵育时，观察到半乳糖神经酰胺完全脱硫。支持我们关于半乳糖神经酰胺脱硫在射精前不会开始这一假设的最重要论据如下：1）在附睾或新鲜射出的精子样本中检测不到脱硫半乳糖神经酰胺；2）由于其细胞内定位，顶体中的芳基硫酸酯酶A无法使完整精子质膜表面的半乳糖神经酰胺脱硫；3）细胞外的芳基硫酸酯酶A储存在精囊中，因此在射精期间和射精后可与精子相互作用。
