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一项关于医院和家庭中制备及使用的肠内营养制剂中细菌数量的对比研究。

A comparative study of the numbers of bacteria present in enteral feeds prepared and administered in hospital and the home.

作者信息

Anderton A, Nwoguh C E, McKune I, Morrison L, Greig M, Clark B

机构信息

Department of Bioscience and Biotechnology, University of Strathclyde, Glasgow, UK.

出版信息

J Hosp Infect. 1993 Jan;23(1):43-9. doi: 10.1016/0195-6701(93)90129-n.

Abstract

Levels and types of bacterial contamination were compared in enteral feeds prepared and administered in hospital or in the home. Samples of feed administered to children suffering from cystic fibrosis were collected for microbiological analysis immediately after preparation, immediately prior to use (unless administered after preparation) and at the end of feeding. No bacteria were detected in 51 (70%) of the 73 feeds sampled on the hospital ward, and in most of the remaining feeds viable counts were less than 10(1) colony-forming units (cfu) ml-1. However, only four (18%) of the feeds sampled in the home were free from bacterial growth and the remaining 18 feeds contained from 10(1) to more than 10(6) cfu ml-1. Bacteria isolated from feeds sampled both in hospital and the home, included Staphylococcus spp., Bacillus spp., viridans and faecal streptococci, Klebsiella spp. and Enterobacter spp. Enterobacter cloacae was isolated from 11 of the 73 hospital feeds and four of the 22 home feeds. Staphylococcus aureus was isolated from five of the home feeds but from none of the hospital feeds. The higher incidence and numbers of bacteria found in home enteral feeds indicate that further, more detailed studies need to be carried out to find the sources and routes of this contamination and devise methods to minimize the problem.

摘要

对在医院或家中制备和使用的肠内营养制剂中的细菌污染水平和类型进行了比较。收集给予囊性纤维化患儿的营养制剂样本,在制备后、使用前(除非制备后立即使用)以及喂养结束时进行微生物分析。在医院病房采集的73份营养制剂样本中,51份(70%)未检测到细菌,其余大多数营养制剂中的活菌数少于10(1) 菌落形成单位(cfu)/毫升。然而,在家中采集的营养制剂样本中,只有4份(18%)没有细菌生长,其余18份含有10(1) 至超过10(6) cfu/毫升的细菌。从医院和家中采集的营养制剂样本中分离出的细菌包括葡萄球菌属、芽孢杆菌属、草绿色链球菌和粪链球菌、克雷伯菌属和肠杆菌属。从73份医院营养制剂中的11份以及22份家庭营养制剂中的4份中分离出阴沟肠杆菌。从5份家庭营养制剂中分离出金黄色葡萄球菌,但医院营养制剂中均未分离出。在家用肠内营养制剂中发现的细菌发生率和数量较高,这表明需要进一步开展更详细的研究,以找出这种污染的来源和途径,并设计方法将问题最小化。

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