Department of Biological and Environmental Sciences, Troy University, Troy, Alabama, USA.
Kinesiology & Health Promotion, Troy University, Troy, Alabama, USA.
Nutr Clin Pract. 2020 Jun;35(3):479-486. doi: 10.1002/ncp.10450. Epub 2020 Jan 29.
The number of patients requiring home enteral nutrition (HEN) continues to increase. Many of these patients are interested in using blended food instead of, or in addition to, commercial enteral formula (CEF). Increased risk of food-borne illness is a concern of blenderized tube-feeding (BTF). This project assessed a standard procedure for minimizing bacterial growth of BTF prepared in the home setting.
Fifty participants prepared BTF in their kitchens using a standard preparation procedure to minimize bacterial contamination. BTF was assessed for growth of aerobic microorganisms, Escherichia coli, Staphylococcus aureus, and coliforms at baseline, 24-hour, and 48-hour intervals after preparation for a total of 150 colony forming units (CFU) counts performed.
No sample had zero aerobic microbial counts; yet no substantial increase in microbial counts was observed during the 48 hours. At baseline and 24 hours, 5/50 (10%) had a CFU count of >10 , and at 48 hours, 6/50 (12%) exceeded 10 CFUs. Out of 150 CFU counts, 2 (1.3%) were just over 10 CFU/mL. Samples exceeding 10 CFU/mL were likely contaminated by common endospore-forming bacteria found in soil or by bacteria in milk that was close to its expiration date.
In this study, 88% of the samples met the US Food Code criteria for safe food consumption; 10.7% met guidelines for marginal safety by other standards; and 1.3% slightly exceeded 10 CFUs. Established safe food-handling procedures can minimize bacterial contamination of BTF and consequently reduce risk of food-borne infection in HEN patients.
需要家庭肠内营养（HEN）的患者数量持续增加。这些患者中有许多人有兴趣使用混合食物代替或补充商业肠内配方（CEF）。 blenderized tube-feeding（BTF）存在食源性疾病风险增加的问题。本项目评估了一种在家中制备 BTF 以最大程度减少细菌生长的标准程序。
50 名参与者在厨房中使用标准制备程序制备 BTF，以最大程度减少细菌污染。在制备后 24 小时和 48 小时，对 BTF 进行需氧微生物、大肠杆菌、金黄色葡萄球菌和大肠菌群生长情况评估，共进行了 150 个菌落形成单位（CFU）计数。
没有样本的需氧微生物计数为零；然而，在 48 小时内没有观察到微生物计数的显著增加。在基线和 24 小时时，5/50（10%）的 CFU 计数>10，而在 48 小时时，6/50（12%）超过 10 CFU。在 150 个 CFU 计数中，有 2 个（1.3%）略高于 10 CFU/mL。超过 10 CFU/mL 的样本可能受到土壤中常见内生孢子形成细菌或接近保质期的牛奶中细菌的污染。
在这项研究中，88%的样本符合美国食品法典关于安全食品消费的标准；10.7%符合其他标准的边缘安全指南；而 1.3%略高于 10 CFU。既定的安全食品处理程序可以最大程度减少 BTF 的细菌污染，从而降低 HEN 患者食源性感染的风险。
