Horiike N, Onji M, Kumon I, Kanaoka M, Michitaka K, Ohta Y
Third Department of Internal Medicine, Ehime University School of Medicine, Japan.
Liver. 1993 Feb;13(1):10-4. doi: 10.1111/j.1600-0676.1993.tb00598.x.
The expression of intercellular adhesion molecule-1 (ICAM-1) on the hepatocyte membrane was studied in 27 patients with chronic hepatitis B and C (CHB, CHC) by immunostaining using a monoclonal antibody. ICAM-1 was expressed focally in a honeycomb-like pattern by hepatocytes in livers of 26/27 patients. The degree of ICAM-1 expression was closely related to the ALT level and the histological grade of liver damage. Abundant cytotoxic T cells (CD8+, CD11b-) were found in ICAM-1-positive areas of the liver. Zones of focal necrosis contained both ICAM-1-positive hepatocytes and cytotoxic T cells. The expression of ICAM-1 was decreased in 4/6 CHB patients after interferon-alpha therapy. No relationship between the degree of hepatocyte ICAM-1 expression and viral replication markers (DNA polymerase activity and the presence of HBcA in the liver) was observed in patients with CHB. In addition, no positive correlation was found between the distribution of ICAM-1-positive hepatocytes and HBcAg-positive hepatocytes. These results suggest that ICAM-1 may play an important role in the pathogenesis of hepatocellular injury mediated by cytotoxic T cells in CHB and CHC.
采用单克隆抗体免疫染色法，对27例慢性乙型和丙型肝炎（CHB、CHC）患者肝细胞膜上细胞间黏附分子-1（ICAM-1）的表达进行了研究。27例患者中有26例患者肝脏中的肝细胞以蜂窝状模式局灶性表达ICAM-1。ICAM-1的表达程度与丙氨酸转氨酶（ALT）水平及肝损伤的组织学分级密切相关。在肝脏ICAM-1阳性区域发现大量细胞毒性T细胞（CD8+、CD11b-）。局灶性坏死区域同时含有ICAM-1阳性肝细胞和细胞毒性T细胞。4/6例CHB患者在接受α干扰素治疗后ICAM-1表达降低。在CHB患者中，未观察到肝细胞ICAM-1表达程度与病毒复制标志物（DNA聚合酶活性及肝脏中HbcA的存在情况）之间存在关联。此外，ICAM-1阳性肝细胞分布与HbcAg阳性肝细胞之间未发现正相关。这些结果表明，ICAM-1可能在CHB和CHC中由细胞毒性T细胞介导的肝细胞损伤发病机制中发挥重要作用。
