Moore D A, Henderson D, Gazzard B G
Academic Department of Immunology, Chelsea & Westminster Hospital, London, UK.
Clin Exp Immunol. 1998 Oct;114(1):73-7. doi: 10.1046/j.1365-2249.1998.00686.x.
Neutrophil dysfunction in HIV disease is well described. We examined the expression of neutrophil adhesion molecules amongst 72 HIV-infected subjects using a whole blood flow cytometric assay with FITC- and R-PE-labelled isotype-specific MoAbs. We report lesser expression of CD11a (LFA-1) and L-selectin (CD62L) on the circulating neutrophils of HIV+ subjects compared with HIV- controls. Expression of CD11b (Mac-1) was unchanged. Shedding of L-selectin and up-regulation of CD11b in response to in vitro stimulation with N-formyl-methionyl-leucyl-phenylalanine (fMLP) were less in HIV+ compared with HIV- subjects, most markedly in subjects with CD4 cell counts < 100 cells/mm3. These results suggest that neutrophil dysfunction in HIV disease, which increases with disease progression, may be attributable to dysregulated adhesion molecule expression.
HIV疾病中的中性粒细胞功能障碍已有充分描述。我们使用FITC和R-PE标记的同型特异性单克隆抗体，通过全血流式细胞术检测了72名HIV感染受试者中性粒细胞黏附分子的表达。我们报告称，与HIV阴性对照组相比，HIV阳性受试者循环中性粒细胞上CD11a（淋巴细胞功能相关抗原-1）和L-选择素（CD62L）的表达较低。CD11b（巨噬细胞抗原-1）的表达没有变化。与HIV阴性受试者相比，HIV阳性受试者在受到N-甲酰甲硫氨酰亮氨酰苯丙氨酸（fMLP）体外刺激后，L-选择素的脱落和CD11b的上调较少，在CD4细胞计数<100个细胞/mm3的受试者中最为明显。这些结果表明，HIV疾病中的中性粒细胞功能障碍随疾病进展而增加，可能归因于黏附分子表达失调。
