Soos J M, Schiffenbauer J, Johnson H M
Department of Microbiology and Cell Science, University of Florida, Gainesville 32611.
J Neuroimmunol. 1993 Mar;43(1-2):39-43. doi: 10.1016/0165-5728(93)90073-8.
Experimental allergic encephalomyelitis (EAE), an antigen induced autoimmune disease, is mediated by V beta 8+ CD4+ T cells in PL/J mice after injection with the autoantigen, myelin basic protein (MBP). Recently the superantigen, staphylococcal enterotoxin B (SEB), has been shown to peripherally anergize and delete T cells in a V beta specific manner. By treatment of PL/J mice with SEB, we have been able to protect PL/J mice from the development of EAE. Two-color FACS analysis of the spleens of SEB treated mice showed depletion of V beta 8+ CD4+ T cells. Consistent with this observation, spleen cells of SEB treated mice that did not show signs of EAE could not be stimulated in vitro with SEB but did respond to SEA. Thus, V beta specific superantigens may prove to be a preventative therapy for autoimmune diseases mediated by V beta specific T lymphocytes.
实验性变应性脑脊髓炎（EAE）是一种抗原诱导的自身免疫性疾病，在PL/J小鼠注射自身抗原髓磷脂碱性蛋白（MBP）后，由Vβ8 + CD4 + T细胞介导。最近研究表明，超抗原葡萄球菌肠毒素B（SEB）能够以Vβ特异性方式使外周T细胞失能并清除。通过用SEB处理PL/J小鼠，我们已能够保护PL/J小鼠不发生EAE。对经SEB处理小鼠的脾脏进行双色荧光激活细胞分选术（FACS）分析显示，Vβ8 + CD4 + T细胞减少。与该观察结果一致，未表现出EAE迹象的经SEB处理小鼠的脾细胞在体外不能被SEB刺激，但对SEA有反应。因此，Vβ特异性超抗原可能被证明是一种针对由Vβ特异性T淋巴细胞介导的自身免疫性疾病的预防性疗法。
