Racke M K, Quigley L, Cannella B, Raine C S, McFarlin D E, Scott D E
Neuroimmunology Branch, National Institute of Neurological Disorders and Stroke, National Institutes of Health, Bethesda, MD 20892.
J Immunol. 1994 Feb 15;152(4):2051-9.
Experimental allergic encephalomyelitis (EAE) is an autoimmune disease that can be induced by the adoptive transfer of CD4, myelin basic protein (MBP)-specific T cells. Superantigens activate T cells expressing appropriate TCR V genes. In this study, MBP-specific T cells activated in vitro with a superantigen, staphylococcal enterotoxin B (SEB), could adoptively transfer a severe form of EAE in (PLxSJL)F1 mice, but did not transfer disease in PL/J or SJL/J mice. SEB treatment of donor mice anergized MBP-specific T cells using V beta 8 in (PLxSJL)F1 mice, because subsequent in vitro activation with SEB resulted in a marked decrease in proliferation to SEB and inability to transfer EAE. However, donor cells from (PLxSJL)F1 mice immunized with MBP/CFA that had been exposed to SEB in vivo before MBP stimulation in vitro still produced EAE in recipient mice. To confirm that non-V beta 8 T cells could transfer disease, donor mice were treated with antibody that eliminated V beta 8 T cells; MBP-activated T cells from these mice could still transfer EAE. Finally, EAE induced by SEB-activated T cells was substantially reduced in mice receiving anti-V beta 8 therapy in vivo. The ability of superantigens to activate encephalitogenic T cells may have relevance to human diseases such as multiple sclerosis.
实验性变应性脑脊髓炎（EAE）是一种自身免疫性疾病，可通过过继转移CD4、髓鞘碱性蛋白（MBP）特异性T细胞诱导产生。超抗原可激活表达适当TCR V基因的T细胞。在本研究中，用超抗原葡萄球菌肠毒素B（SEB）体外激活的MBP特异性T细胞，可在（PLxSJL）F1小鼠中过继转移严重形式的EAE，但在PL/J或SJL/J小鼠中不能转移疾病。在（PLxSJL）F1小鼠中，SEB处理供体小鼠可使利用Vβ8的MBP特异性T细胞失能，因为随后用SEB进行体外激活会导致对SEB的增殖显著降低且无法转移EAE。然而，来自（PLxSJL）F1小鼠的供体细胞，在体外MBP刺激前已在体内接触过SEB的情况下，用MBP/完全弗氏佐剂免疫后，仍能在受体小鼠中产生EAE。为证实非Vβ8 T细胞可转移疾病，用消除Vβ8 T细胞的抗体处理供体小鼠；来自这些小鼠的MBP激活的T细胞仍可转移EAE。最后，在体内接受抗Vβ8治疗的小鼠中，由SEB激活的T细胞诱导的EAE显著减少。超抗原激活致脑炎性T细胞的能力可能与诸如多发性硬化症等人类疾病相关。
