Koiwai O, Yasui Y, Sakai Y, Watanabe T, Ishii K, Yanagihara S, Andoh T
Laboratory of Biochemistry, Aichi Cancer Center Research Institute, Nagoya, Japan.
Gene. 1993 Mar 30;125(2):211-6. doi: 10.1016/0378-1119(93)90331-v.
The mouse cDNA encoding DNA topoisomerase I (TopoI) was cloned and the nucleotide sequence of 3512 bp was determined. The cDNA clone contained an open reading frame encoding a protein of 767 amino acids (aa), which is 2 aa longer than its human counterpart. Overall aa sequence homology between the mouse and human, and between the mouse and yeast (Saccharomyces cerevisiae) sequences was 96% and 42%, respectively. The mouse TopI gene was mapped at position 54.5 on chromosome 2 from linkage analyses of a three-point cross test with Geg, Ada, and a as marker genes.
