Casiano C A, Tan E M
W.M. Keck Autoimmune Disease Center, Department of Molecular and Experimental Medicine SBR6, Scripps Research Institute, La Jolla, CA 92037, USA.
Mol Biol Rep. 1996;23(3-4):211-6. doi: 10.1007/BF00351171.
Antinuclear autoantibodies (ANAs) derived from patients with systemic autoimmune diseases have proven to be powerful tools in cell and molecular biology, The availability of these autoantibodies has been instrumental in the identification and characterization of a wide range of intracellular proteins involved in essential cellular activities. Recently, these autoantibodies have been used in molecular studies of apoptosis, particularly in the identification of substrates cleaved by proteases of the ICE/CED-3 family during this cell death pathway. The identification of these substrates may help to understand the role of proteolysis in apoptosis. Examples of nuclear autoantigens whose cleavage during apoptosis have been defined using ANAs include the 70 kD protein of the U1 small nuclear ribonucleoprotein particle (U1-70 kD), the nuclear mitotic apparatus protein (NuMA), DNA topoisomerase I, the RNA polymerase I upstream binding factor (UBF), and the catalytic subunit of DNA-dependent protein kinase (DNA-PKcs). The use of ANAs as probes for defining proteolytic events associated with apoptosis promises to yield important insights into the mechanisms driving this cell death pathway.
源自系统性自身免疫疾病患者的抗核自身抗体（ANA）已被证明是细胞和分子生物学中的有力工具。这些自身抗体的可得性有助于鉴定和表征参与基本细胞活动的多种细胞内蛋白质。最近，这些自身抗体已用于细胞凋亡的分子研究，特别是在鉴定该细胞死亡途径中ICE/CED-3家族蛋白酶切割的底物方面。这些底物的鉴定可能有助于理解蛋白水解在细胞凋亡中的作用。使用ANA确定凋亡过程中发生切割的核自身抗原的例子包括U1小核核糖核蛋白颗粒的70kD蛋白（U1-70kD）、核有丝分裂器蛋白（NuMA）、DNA拓扑异构酶I、RNA聚合酶I上游结合因子（UBF）以及DNA依赖性蛋白激酶的催化亚基（DNA-PKcs）。将ANA用作定义与细胞凋亡相关的蛋白水解事件的探针，有望为驱动该细胞死亡途径的机制提供重要见解。
