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Restriction fragment length polymorphism analysis of Fusobacterium necrophorum using a novel repeat DNA sequence and a 16S rRNA gene probe.

作者信息

Hodgson A L, Nicholson L A, Doran T J, Corner L A

机构信息

CSIRO Division of Animal Health, Animal Health Research Laboratory, Parkville, Vic., Australia.

出版信息

FEMS Microbiol Lett. 1993 Mar 1;107(2-3):205-10. doi: 10.1111/j.1574-6968.1993.tb06031.x.

DOI:10.1111/j.1574-6968.1993.tb06031.x
PMID:8097174
Abstract

A repeated DNA sequence was isolated from Fusobacterium necrophorum biotype AB, strain FnS1. The repeated sequence shared considerable homology with the transposase gene from the Pseudomonas syringiae insertion sequence IS801. The repeat sequence was used together with a 16S ribosomal RNA gene probe to type F. necrophorum isolates using restriction fragment length polymorphisms. The probes revealed differences between several clinical isolates and will be useful tools to study the epidemiology of ovine foot abscess and other diseases caused by F. necrophorum.

摘要

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引用本文的文献

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Molecular diagnosis of Fusobacterium necrophorum infection (Lemierre's syndrome).坏死梭杆菌感染(勒米尔综合征)的分子诊断
Eur J Clin Microbiol Infect Dis. 2005 Mar;24(3):226-9. doi: 10.1007/s10096-005-1298-6.
2
Insertion sequences.插入序列
Microbiol Mol Biol Rev. 1998 Sep;62(3):725-74. doi: 10.1128/MMBR.62.3.725-774.1998.
3
Ribotyping to compare Fusobacterium necrophorum isolates from bovine liver abscesses, ruminal walls, and ruminal contents.采用核糖体分型法比较从牛肝脓肿、瘤胃壁和瘤胃内容物中分离出的坏死梭杆菌菌株。
Appl Environ Microbiol. 1997 Dec;63(12):4671-8. doi: 10.1128/aem.63.12.4671-4678.1997.
4
Ribotyping to differentiate Fusobacterium necrophorum subsp. necrophorum and F. necrophorum subsp. funduliforme isolated from bovine ruminal contents and liver abscesses.利用核糖体分型法鉴别从牛瘤胃内容物和肝脓肿中分离出的坏死梭杆菌坏死亚种和坏死梭杆菌底形亚种。
Appl Environ Microbiol. 1996 Feb;62(2):469-72. doi: 10.1128/aem.62.2.469-472.1996.