关于外源细菌DNA整合到植物DNA中的问题。
On the question of the integration of exogenous bacterial DNA into plant DNA.
作者信息
Kleinhofs A, Eden F C, Chilton M D, Bendich A J
出版信息
Proc Natl Acad Sci U S A. 1975 Jul;72(7):2748-52. doi: 10.1073/pnas.72.7.2748.
Extensive studies with pea, tomato, and barley failed to confirm the evidence presented by previous investigators for integration or replication of exogenously applied bacterial DNA in these plants. Labeled DNA of buoyant density in CsCl intermediate between that of high density donor bacterial DNA and of plant DNA was never observed with axenic plants. Intermediate peaks, similar to those used as evidence for recombination by earlier investigators, were observed only when the plants were contaminated with bacteria. Plant DNA prepared by a published procedure [Ledoux, L. & Huart, R. (1969) J. Mol. Biol. 43, 243-262] was found to be contaminated with unidentified impurities. Such DNA was partially protected from the action of DNase and produced aberrant banding patterns in CsCl after shearing. Much of the published evidence for integration of foreign DNA in plants is based upon experiments with plant DNA prepared by this procedure. We conclude that contamination is the likely explanation for what has been interpreted as evidence for integration.
对豌豆、番茄和大麦进行的广泛研究未能证实先前研究人员所提出的关于外源施加的细菌DNA在这些植物中整合或复制的证据。在用无菌植物进行的实验中，从未观察到在氯化铯中浮力密度介于高密度供体细菌DNA和植物DNA之间的标记DNA。只有当植物被细菌污染时，才会观察到类似于早期研究人员用作重组证据的中间峰。通过已发表的程序制备的植物DNA [勒杜，L. & 于阿尔特，R. (1969) 《分子生物学杂志》43, 243 - 262] 被发现含有未鉴定的杂质。这种DNA在一定程度上受到脱氧核糖核酸酶作用的保护，并且在剪切后在氯化铯中产生异常的条带模式。许多已发表的关于外源DNA在植物中整合的证据是基于用此程序制备的植物DNA所进行的实验。我们得出结论，污染可能是被解释为整合证据的现象的原因。
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