Matsui T, Hirai M, Wakita M, Hirano M, Kurosawa Y
Institute for Comprehensive Medical Science, Fujita Health University, Aichi, Japan.
FEBS Lett. 1993 May 10;322(2):181-5. doi: 10.1016/0014-5793(93)81564-g.
A homeobox is a DNA sequence of 180 base pairs that encodes a DNA-binding domain known as a homeodomain. The polymerase chain reaction (PCR) has been used to prepare probes of homeobox-containing genes. We cloned and sequenced the amplified products of PCR that was performed with human genomic DNA and two primers that correspond to well-conserved regions in homeoboxes. Fifteen kinds of homeobox gene were identified and 13 of them were assigned to HOX genes that have already been reported. Two others represented novel homeobox genes and one of them, GBX1, was mapped to chromosome 7q36.1 by fluorescence in situ hybridization. Northern hybridization of mRNA for various kinds of hematopoietic cell showed that the newly identified GBX1 gene is expressed in K562 cells and Daudi cells.
同源异型框是一段由180个碱基对组成的DNA序列，它编码一种被称为同源异型结构域的DNA结合结构域。聚合酶链反应（PCR）已被用于制备含同源异型框基因的探针。我们克隆并测序了用人类基因组DNA以及与同源异型框中保守区域相对应的两种引物进行PCR扩增得到的产物。鉴定出了15种同源异型框基因，其中13种被归类为已报道的HOX基因。另外两种代表新的同源异型框基因，其中之一GBX1通过荧光原位杂交被定位到7号染色体的7q36.1区域。对各种造血细胞的mRNA进行Northern杂交显示，新鉴定出的GBX1基因在K562细胞和Daudi细胞中表达。
