Bates E J, Ferrante A, Harvey D P, Poulos A
Department of Immunology, Women's and Children's Hospital, Adelaide, Australia.
J Leukoc Biol. 1993 Apr;53(4):420-6. doi: 10.1002/jlb.53.4.420.
Fish oils are abundant in polyunsaturated fatty acids of the n-3 series (in particular eicosapentaenoic, 20:5 and docosahexaenoic acid, 22:6). Such fatty acids are generally considered to be beneficial in the prevention of cardiac disease and to have anti-inflammatory properties. Neutrophil adherence is an essential early event in an acute inflammatory response, and we have demonstrated that both 20:5 and 22:6 stimulate adherence in vitro. Arachidonic acid (20:4, n-6) was also stimulatory. Significant simulation of adherence was seen from 5 to 80 microM (nontoxic concentrations) 22:6, 20:5, or 20:4. At the lower fatty acid concentrations tested (< or = 40 microM) 20:5 was less active than 22:6 or 20:4 at stimulating adherence. Above 40 microM there was no difference in the ability of the three fatty acids to stimulate adherence. At the lower fatty acid concentrations tested (< or = 10 microM) 22:6 was less active than 20:4, whereas above 10 microM they were equally active. Immunofluorescent flow cytometric analysis of neutrophil integrin (adherence) receptors showed that the complement C3bi receptor (CD11b) was up-regulated by these fatty acids. There was no change in CD11a or CD11c. Saturated fatty acids of the same chain length were without effect on adherence or receptor expression. The findings suggest that these polyunsaturated fatty acids may, under certain conditions, be proinflammatory with respect to their acute effects on the interaction of neutrophils with microbes, endothelium, and other tissues.
鱼油富含n-3系列多不饱和脂肪酸（特别是二十碳五烯酸，20:5和二十二碳六烯酸，22:6）。这类脂肪酸通常被认为对预防心脏病有益且具有抗炎特性。中性粒细胞黏附是急性炎症反应中一个重要的早期事件，并且我们已经证明20:5和22:6在体外均能刺激黏附。花生四烯酸（20:4，n-6）也具有刺激作用。在5至80微摩尔（无毒浓度）的22:6、20:5或20:4时，观察到了显著的黏附刺激作用。在测试的较低脂肪酸浓度（≤40微摩尔）下，20:5在刺激黏附方面的活性低于22:6或20:4。在40微摩尔以上，这三种脂肪酸刺激黏附的能力没有差异。在测试的较低脂肪酸浓度（≤10微摩尔）下，22:6的活性低于20:4，而在10微摩尔以上它们的活性相当。对中性粒细胞整合素（黏附）受体的免疫荧光流式细胞术分析表明，这些脂肪酸上调了补体C3bi受体（CD11b）。CD11a或CD11c没有变化。相同链长的饱和脂肪酸对黏附或受体表达没有影响。这些发现表明，这些多不饱和脂肪酸在某些条件下，就其对中性粒细胞与微生物、内皮细胞及其他组织相互作用的急性影响而言，可能具有促炎作用。
