乙酸激酶介导的大肠杆菌鞭毛旋转方向的改变
Change in direction of flagellar rotation in Escherichia coli mediated by acetate kinase.
作者信息
Dailey F E, Berg H C
机构信息
Department of Cellular and Developmental Biology, Harvard University, Cambridge, Massachusetts 02138.
出版信息
J Bacteriol. 1993 May;175(10):3236-9. doi: 10.1128/jb.175.10.3236-3239.1993.
Abstract
Strains of Escherichia coli lacking all cytoplasmic chemotaxis proteins except CheY swim smoothly under most conditions. However, they tumble when exposed to acetate. Acetate coenzyme A synthetase (EC 6.2.1.1) was thought to be essential for this response. New evidence suggests that the tumbling is mediated instead by acetate kinase (EC 2.7.2.1), which might phosphorylate CheY via acetyl phosphate. In strains that were wild type for chemotaxis, neither acetate coenzyme A synthetase, acetate kinase, nor phosphotransacetylase (EC 2.3.1.8) (and thus acetyl phosphate) was required for responses to aspartate, serine, or sugars sensed by the phosphotransferase system. Thus, acetate-induced tumbling does not appear to play an essential role in chemotaxis in wild-type cells.
摘要
除CheY外缺乏所有细胞质趋化蛋白的大肠杆菌菌株在大多数情况下游动顺畅。然而，当暴露于乙酸盐时它们会翻滚。乙酸辅酶A合成酶（EC 6.2.1.1）被认为对这种反应至关重要。新证据表明，翻滚反应相反是由乙酸激酶（EC 2.7.2.1）介导的，乙酸激酶可能通过乙酰磷酸使CheY磷酸化。在趋化性为野生型的菌株中，对天冬氨酸、丝氨酸或磷酸转移酶系统感知的糖类的反应，既不需要乙酸辅酶A合成酶、乙酸激酶，也不需要磷酸转乙酰酶（EC 2.3.1.8）（以及因此的乙酰磷酸）。因此，乙酸诱导的翻滚似乎在野生型细胞的趋化性中不发挥重要作用。
相似文献
1
Change in direction of flagellar rotation in Escherichia coli mediated by acetate kinase.乙酸激酶介导的大肠杆菌鞭毛旋转方向的改变
J Bacteriol. 1993 May;175(10):3236-9. doi: 10.1128/jb.175.10.3236-3239.1993.
2
Both acetate kinase and acetyl coenzyme A synthetase are involved in acetate-stimulated change in the direction of flagellar rotation in Escherichia coli.乙酸激酶和乙酰辅酶A合成酶都参与了乙酸刺激的大肠杆菌鞭毛旋转方向的改变。
J Bacteriol. 1998 Feb;180(4):985-8. doi: 10.1128/JB.180.4.985-988.1998.
3
Acetylation at Lys-92 enhances signaling by the chemotaxis response regulator protein CheY.赖氨酸92位的乙酰化增强了趋化反应调节蛋白CheY的信号传导。
Proc Natl Acad Sci U S A. 1998 Apr 28;95(9):4918-23. doi: 10.1073/pnas.95.9.4918.
4
Co-regulation of acetylation and phosphorylation of CheY, a response regulator in chemotaxis of Escherichia coli.大肠杆菌趋化作用中响应调节因子CheY的乙酰化和磷酸化的共同调节。
J Mol Biol. 2004 Sep 10;342(2):375-81. doi: 10.1016/j.jmb.2004.07.021.
5
The enzymic interconversion of acetate and acetyl-coenzyme A in Escherichia coli.大肠杆菌中乙酸盐与乙酰辅酶A的酶促相互转化
J Gen Microbiol. 1977 Oct;102(2):327-36. doi: 10.1099/00221287-102-2-327.
6
Cloning, characterization, and functional expression of acs, the gene which encodes acetyl coenzyme A synthetase in Escherichia coli.大肠杆菌中编码乙酰辅酶A合成酶的基因acs的克隆、特性分析及功能表达
J Bacteriol. 1995 May;177(10):2878-86. doi: 10.1128/jb.177.10.2878-2886.1995.
7
Acetate kinase and phosphotransacetylase.乙酸激酶和磷酸转乙酰酶。
Methods Enzymol. 2011;494:219-31. doi: 10.1016/B978-0-12-385112-3.00011-1.
8
Acetyladenylate plays a role in controlling the direction of flagellar rotation.乙酰腺苷酸在控制鞭毛旋转方向中起作用。
Proc Natl Acad Sci U S A. 1988 Sep;85(18):6711-5. doi: 10.1073/pnas.85.18.6711.
9
Roles of cheY and cheZ gene products in controlling flagellar rotation in bacterial chemotaxis of Escherichia coli.cheY和cheZ基因产物在大肠杆菌细菌趋化性中控制鞭毛旋转的作用。
J Bacteriol. 1987 Mar;169(3):1307-14. doi: 10.1128/jb.169.3.1307-1314.1987.
10
Acetylation of the chemotaxis response regulator CheY by acetyl-CoA synthetase purified from Escherichia coli.从大肠杆菌中纯化的乙酰辅酶A合成酶对趋化反应调节因子CheY的乙酰化作用。
J Mol Biol. 2004 Sep 10;342(2):383-401. doi: 10.1016/j.jmb.2004.07.020.
引用本文的文献
1
Interrogating the Transient Selectivity of Bacterial Chemotaxis-Driven Affinity and Accumulation of Carbonaceous Substances Raman Microspectroscopy.探究细菌趋化性驱动的碳质物质亲和力和积累的瞬态选择性：拉曼光谱。
Front Microbiol. 2019 Oct 4;10:2215. doi: 10.3389/fmicb.2019.02215. eCollection 2019.
2
Targeting Bacterial Dsb Proteins for the Development of Anti-Virulence Agents.靶向细菌二硫键形成蛋白以开发抗毒力剂
Molecules. 2016 Jul 16;21(7):811. doi: 10.3390/molecules21070811.
3
The ArcB leucine zipper domain is required for proper ArcB signaling.ArcB 的亮氨酸拉链结构域对于正确的 ArcB 信号传递是必需的。
PLoS One. 2012;7(5):e38187. doi: 10.1371/journal.pone.0038187. Epub 2012 May 30.
4
An insight into the role of phosphotransacetylase (pta) and the acetate/acetyl-CoA node in Escherichia coli.深入了解磷酸转乙酰酶（pta）和乙酸盐/乙酰辅酶A节点在大肠杆菌中的作用。
Microb Cell Fact. 2009 Oct 24;8:54. doi: 10.1186/1475-2859-8-54.
5
Evidence against the physiological role of acetyl phosphate in the phosphorylation of the ArcA response regulator in Escherichia coli.关于磷酸乙酰在大肠杆菌ArcA反应调节因子磷酸化过程中生理作用的反证。
J Microbiol. 2009 Oct;47(5):657-62. doi: 10.1007/s12275-009-0087-9. Epub 2009 Oct 24.
6
Requirement of the receiver and phosphotransfer domains of ArcB for efficient dephosphorylation of phosphorylated ArcA in vivo.ArcB的受体和磷酸转移结构域在体内对磷酸化的ArcA进行有效去磷酸化的需求。
J Bacteriol. 2005 May;187(9):3267-72. doi: 10.1128/JB.187.9.3267-3272.2005.
7
Characterization of the acetate binding pocket in the Methanosarcina thermophila acetate kinase.嗜热甲烷八叠球菌乙酸激酶中乙酸结合口袋的表征
J Bacteriol. 2005 Apr;187(7):2386-94. doi: 10.1128/JB.187.7.2386-2394.2005.
8
The acetate switch.乙酸盐转换
Microbiol Mol Biol Rev. 2005 Mar;69(1):12-50. doi: 10.1128/MMBR.69.1.12-50.2005.
9
Repression of Escherichia coli PhoP-PhoQ signaling by acetate reveals a regulatory role for acetyl coenzyme A.乙酸盐对大肠杆菌PhoP-PhoQ信号传导的抑制揭示了乙酰辅酶A的调节作用。
J Bacteriol. 2003 Apr;185(8):2563-70. doi: 10.1128/JB.185.8.2563-2570.2003.
10
Acetylation at Lys-92 enhances signaling by the chemotaxis response regulator protein CheY.赖氨酸92位的乙酰化增强了趋化反应调节蛋白CheY的信号传导。
Proc Natl Acad Sci U S A. 1998 Apr 28;95(9):4918-23. doi: 10.1073/pnas.95.9.4918.
本文引用的文献
1
Acyl adenylates; an enzymatic mechanism of acetate activation.酰基腺苷酸；乙酸激活的一种酶促机制。
J Biol Chem. 1956 Oct;222(2):991-1013.
2
Enzymatic phosphorylation of acetate.乙酸的酶促磷酸化作用。
J Biol Chem. 1954 Dec;211(2):737-56.
3
Selection for loss of tetracycline resistance by Escherichia coli.大肠杆菌对四环素抗性丧失的选择。
J Bacteriol. 1981 Feb;145(2):1110-1. doi: 10.1128/jb.145.2.1110-1111.1981.
4
Isolation and Characterization of acetate kinase and phosphotransacetylase mutants of Escherichia coli and Salmonella typhimurium.大肠杆菌和鼠伤寒沙门氏菌乙酸激酶及磷酸转乙酰酶突变体的分离与鉴定
J Bacteriol. 1980 Aug;143(2):1081-5. doi: 10.1128/jb.143.2.1081-1085.1980.
5
Overexpression and sequence of the Escherichia coli cheY gene and biochemical activities of the CheY protein.大肠杆菌cheY基因的过表达、序列及CheY蛋白的生化活性
J Bacteriol. 1984 Oct;160(1):36-41. doi: 10.1128/jb.160.1.36-41.1984.
6
Nonchemotactic mutants of Escherichia coli.大肠杆菌的非趋化性突变体。
J Bacteriol. 1967 Jan;93(1):390-8. doi: 10.1128/jb.93.1.390-398.1967.
7
Reconstitution of signaling in bacterial chemotaxis.细菌趋化作用中信号的重构。
J Bacteriol. 1987 May;169(5):1878-85. doi: 10.1128/jb.169.5.1878-1885.1987.
8
Phosphorylation of three proteins in the signaling pathway of bacterial chemotaxis.细菌趋化信号通路中三种蛋白质的磷酸化作用。
Cell. 1988 Apr 8;53(1):79-87. doi: 10.1016/0092-8674(88)90489-8.
9
Phosphate transfer between acetate kinase and enzyme I of the bacterial phosphotransferase system.细菌磷酸转移酶系统中乙酸激酶与酶I之间的磷酸转移
J Biol Chem. 1986 Oct 15;261(29):13498-503.
10
Acetyladenylate plays a role in controlling the direction of flagellar rotation.乙酰腺苷酸在控制鞭毛旋转方向中起作用。
Proc Natl Acad Sci U S A. 1988 Sep;85(18):6711-5. doi: 10.1073/pnas.85.18.6711.
Zotero 插件