六种动物血小板中的γ-氨基丁酸转氨酶活性

gamma-Aminobutyrate aminotransferase activity in blood platelets of six species.

Sherif F M, Jossan S S, Oreland L

Department of Medical Pharmacology, University of Uppsala, Sweden.

Comp Biochem Physiol C Comp Pharmacol Toxicol. 1993 Feb;104(2):345-9. doi: 10.1016/0742-8413(93)90046-n.

Abstract

The kinetic parameters of gamma-aminobutyrate aminotransferase (GABA-T) were studied in washed blood platelets from cat, dog, horse, man, mouse and rat. 2. Wide differences were found in the maximal reaction velocity (Vmax) of GABA-T in blood platelets of the six species. 3. A significant increase in the activity of GABA-T was found in the blood platelets of adult rats as compared to those of young rats. 4. However, there was no significant difference in the activity of platelet GABA-T between male and female rats. 5. The activity of human platelet GABA-T estimated in platelet-rich plasma from healthy male subjects was found to be decreased in the presence of plasma and albumin. 6. The decrease was due to the specific binding of the cofactor pyridoxal phosphate to plasma albumin.

摘要

对猫、狗、马、人、小鼠和大鼠的洗涤血小板中γ-氨基丁酸转氨酶（GABA-T）的动力学参数进行了研究。2. 发现这六个物种的血小板中GABA-T的最大反应速度（Vmax）存在很大差异。3. 与幼鼠相比，成年大鼠血小板中GABA-T的活性显著增加。4. 然而，雄性和雌性大鼠的血小板GABA-T活性没有显著差异。5. 发现在含有血浆和白蛋白的情况下，健康男性受试者富含血小板血浆中估算的人血小板GABA-T活性降低。6. 这种降低是由于辅因子磷酸吡哆醛与血浆白蛋白的特异性结合。