Nelson J W, Parcells A J, Cosimi A B, Esser R E
Transplantation. 1975 Jul;20(1):3-12. doi: 10.1097/00007890-197507000-00002.
The rosette inhibition assay for immunosuppressive activity of antilymphocyte globulin or plasma has been studied in an effort to improve its reliability. Important changes include the elimination of calcium and magnesium ions from salt solutions used in the assay, the use of deoxyribonuclease to prevent lymphocyte clumping, and pretreatment of plasma samples (heating at 63 C for 10 min followed by acrinol precipitation) to prevent nonspecific inhibition of rosette formation. The use of a graded dose response potency assay against a house standard is discussed. A significant correlation was established between the in vitro activity of several series of antilymphocyte globulin or antithymocyte globulin preparations and their ability to prolong skin graft survival in primates. The best correlation was achieved using a potency estimate relative to a house standard, rather than the conventional titer estimate.
为提高抗淋巴细胞球蛋白或血浆免疫抑制活性的玫瑰花结抑制试验的可靠性，已对其进行了研究。重要的改进包括：在试验所用盐溶液中去除钙和镁离子；使用脱氧核糖核酸酶防止淋巴细胞聚集；对血浆样本进行预处理（63℃加热10分钟，然后用吖啶黄沉淀）以防止对玫瑰花结形成的非特异性抑制。讨论了针对内部标准品使用分级剂量反应效价测定法。已确定几组抗淋巴细胞球蛋白或抗胸腺细胞球蛋白制剂的体外活性与其在灵长类动物中延长皮肤移植存活时间的能力之间存在显著相关性。相对于内部标准品使用效价估计值，而非传统的滴度估计值，可实现最佳相关性。
