Calligaro A, Ceriani T, Rindi G, Schmid R, Stefanelli M, Ventura U
Arch Int Physiol Biochim. 1978 May;86(2):327-42. doi: 10.3109/13813457809069908.
A five-compartment linear model for diffusion in vitro across rat jejunum has been proposed for the study of the kinetic constants of D-histidine transport. Once preliminary experiments using 2,4-dinitrophenol and L-methionine have proved that D-histidine gives rise to passive transport only, the validity of the model was tested and its parameters estimated through a best-fitting procedure by using experimental data concerning D-histidine transport. D-Histidine diffusion was studied in everted and unreverted loops mounted in an oxygenated bath system. Both mucosa to serosa and seroa to mucosa movements of D-histidine (3-30 mM) were evaluated by measuring chemically the amount of D-histidine transported into intestinal lumen every 5 min for 60 min. Results obtained proved that D-histidine transport in each direction (mucosa to seroa or seroa to mucosa) was dependent-concentration process. Nevertheless different values of gain and time constants were estimated for the transport in the two directions.
为了研究D-组氨酸转运的动力学常数，已提出一种用于体外大鼠空肠扩散的五室线性模型。一旦使用2,4-二硝基苯酚和L-甲硫氨酸进行的初步实验证明D-组氨酸仅产生被动转运，就通过使用关于D-组氨酸转运的实验数据，通过最佳拟合程序测试了模型的有效性并估计了其参数。在安装在充氧浴系统中的外翻和未翻转肠袢中研究了D-组氨酸的扩散。通过化学测量每5分钟转运到肠腔中的D-组氨酸量，持续60分钟，评估了D-组氨酸（3-30 mM）从粘膜到浆膜和从浆膜到粘膜的转运。获得的结果证明，D-组氨酸在每个方向（粘膜到浆膜或浆膜到粘膜）的转运是浓度依赖性过程。然而，在两个方向上的转运估计出了不同的增益值和时间常数。
