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山羊乙酰辅酶A羧化酶互补DNA的分离以及挤奶频率对山羊乳腺中乙酰辅酶A羧化酶和脂肪酸合酶基因表达的影响。

Isolation of a goat acetyl-CoA carboxylase complementary DNA and effect of milking frequency on the expression of the acetyl-CoA carboxylase and fatty acid synthase genes in goat mammary gland.

作者信息

Travers M T, Barber M C

机构信息

Hannah Research Institute, Scotland, U.K.

出版信息

Comp Biochem Physiol B. 1993 May;105(1):123-8. doi: 10.1016/0305-0491(93)90178-8.

DOI:10.1016/0305-0491(93)90178-8
PMID:8099321
Abstract
  1. Using the polymerase chain reaction we have isolated a partial complementary DNA for goat acetyl-CoA carboxylase which is 90 and 82% homologous to the published rat and chicken complementary DNA sequences, respectively. 2. Frequent milking causes an upregulation of the acetyl-CoA carboxylase and fatty acid synthase genes in goat mammary gland that parallels the increase in the respective enzyme activities. 3. The sequence for goat acetyl-CoA carboxylase is in the EMBL data base, Accession Number Z17803.
摘要
  1. 利用聚合酶链反应,我们分离出了山羊乙酰辅酶A羧化酶的部分互补DNA,它与已发表的大鼠和鸡的互补DNA序列分别具有90%和82%的同源性。2. 频繁挤奶会导致山羊乳腺中乙酰辅酶A羧化酶和脂肪酸合酶基因上调,这与相应酶活性的增加同步。3. 山羊乙酰辅酶A羧化酶的序列在EMBL数据库中,登录号为Z17803。

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Evidence that the medium-chain acyltransferase of lactating-goat mammary-gland fatty acid synthetase is identical with the acetyl/malonyltransferase.泌乳山羊乳腺脂肪酸合成酶的中链酰基转移酶与乙酰/丙二酸单酰转移酶相同的证据。
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Acetyl-CoA carboxylase from rat mammary gland (including an additional step yielding fatty acid synthase).来自大鼠乳腺的乙酰辅酶A羧化酶(包括产生脂肪酸合酶的额外步骤)。
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