Kusunoki T, Nakano T, Funasaka K, Murata K, Nishida S, Tomura T, Hashimoto S, Ohta F
Department of Otolaryngology, Kinki University School of Medicine, Osaka.
Nihon Jibiinkoka Gakkai Kaiho. 1993 Apr;96(4):651-8. doi: 10.3950/jibiinkoka.96.651.
Surgical materials obtained from 5 normal human thyroids and 19 diseased tissues (4 with Grave's disease, 4 adenomatous goiters, 3 follicular adenomas, 2 papillary carcinomas, 4 follicular carcinomas and 2 undifferentiated carcinomas) were investigated immunohistochemically using the avidin-biotin-peroxidase complex technique with anti-proliferating cell nuclear antigen (PCNA) antibody and epidermal growth factor receptor (EGFR) antibody. The results of this study suggested the following: (1) Labeling indexes of PCNA positive cell nuclei were shown in high grade malignant cases accompanied by capsular invasion and lymph node metastasis, in undifferentiated carcinoma cells, and in proliferative lesions of benign diseases. These results suggest that PCNA is indicative of proliferative ability in thyroid diseases. (2) On thyroid tumors, EGFR regions with strong positive staining showed many PCNA positive cell nuclei. EGF appears to have a significant association with proliferative ability in thyroid diseases.
采用抗增殖细胞核抗原（PCNA）抗体和表皮生长因子受体（EGFR）抗体，运用抗生物素蛋白-生物素-过氧化物酶复合物技术，对取自5例正常人体甲状腺和19例病变组织（4例格雷夫斯病、4例腺瘤性甲状腺肿、3例滤泡性腺瘤、2例乳头状癌、4例滤泡状癌和2例未分化癌）的手术材料进行免疫组织化学研究。本研究结果提示如下：（1）PCNA阳性细胞核的标记指数在伴有包膜侵犯和淋巴结转移的高级别恶性病例、未分化癌细胞以及良性疾病的增殖性病变中均有显示。这些结果表明PCNA可指示甲状腺疾病中的增殖能力。（2）在甲状腺肿瘤上，强阳性染色的EGFR区域显示有许多PCNA阳性细胞核。表皮生长因子似乎与甲状腺疾病中的增殖能力有显著关联。
