Dawson D A
Department of Animal Science, College of Veterinary Medicine, University of Tennessee, Knoxville 37901-1071.
Teratog Carcinog Mutagen. 1993;13(1):23-33. doi: 10.1002/tcm.1770130104.
A developed mixture testing protocol, evaluating malformation of Xenopus embryos, was used to examine the joint actions of semicarbazide (SC) and thiosemicarbazide (TSC) with benzoic hydrazide (BZH). These compounds were selected to evaluate the effect of a substitution of sulfur for oxygen on osteolathyrism induced in Xenopus embryos. Binary combinations of SC with BZH and TSC with BZH were tested using three mixture ratios for each combination. Each compound was simultaneously tested alone in 96 h static-renewal tests, starting with late-blastula stage embryos. Toxic unit analysis, evaluating induction of malformations in general and osteolathyrism specifically, indicated the joint action for each combination was concentration addition. The results indicate that the three chemicals are similar and non-interactive and are likely to induce osteolathyrism by the same primary mechanism of action.
采用一种成熟的混合物测试方案（用于评估非洲爪蟾胚胎的畸形情况）来检测氨基脲（SC）、氨基硫脲（TSC）与苯甲酰肼（BZH）的联合作用。选择这些化合物是为了评估用硫取代氧对非洲爪蟾胚胎诱导的骨软化病的影响。SC与BZH以及TSC与BZH的二元组合使用每种组合的三种混合比例进行测试。每种化合物在从囊胚晚期胚胎开始的96小时静态更新试验中同时单独进行测试。毒性单位分析（总体评估畸形诱导情况，特别是骨软化病诱导情况）表明，每种组合的联合作用为浓度相加。结果表明，这三种化学物质相似且无相互作用，并且可能通过相同的主要作用机制诱导骨软化病。
