Dawson D A
Department of Animal Science, College of Veterinary Medicine, University of Tennessee, Knoxville 37901-1071.
Toxicology. 1993 Jul 28;81(2):123-30. doi: 10.1016/0300-483x(93)90004-c.
Binary mixtures of the osteolathyrogens benzoic hydrazide and beta-aminopropionitrile were tested to determine the joint action for these chemicals, by evaluating induction of malformation and osteolathyrism in developing frog embryos. Exposures, starting with late-blastula stage embryos, were for 96-h with solution renewal every 24-h. Five treatments were examined, each chemical alone (i.e., 1:0 and 0:1 solutions) and three mixtures (i.e., 3:1, 1:1, and 1:3). Following exposure, embryos were evaluated grossly and the incidences of all types of malformations and osteolathyrogenic lesions were determined. Toxic unit analysis and isobole diagrams indicated these chemicals were concentration additive in inducing malformations and osteolathyrism. Therefore, benzoic hydrazide and beta-aminopropionitrile induce osteolathyrism, in Xenopus embryos, in a similar manner.
通过评估发育中的青蛙胚胎中畸形和骨生成异常的诱导情况，对致骨生成异常剂苯甲酰肼和β-氨基丙腈的二元混合物进行了测试，以确定这些化学物质的联合作用。从囊胚后期胚胎开始暴露，暴露96小时，每24小时更换一次溶液。检查了五种处理，每种化学物质单独使用（即1:0和0:1溶液）以及三种混合物（即3:1、1:1和1:3）。暴露后，对胚胎进行大体评估，并确定所有类型畸形和骨生成异常病变的发生率。毒性单位分析和等效线图表明，这些化学物质在诱导畸形和骨生成异常方面具有浓度相加作用。因此，苯甲酰肼和β-氨基丙腈在非洲爪蟾胚胎中以相似的方式诱导骨生成异常。
