Dawson D A, Wilke T S
University of Tennessee, College of Veterinary Medicine, Knoxville 37901-1071.
J Appl Toxicol. 1991 Dec;11(6):415-21. doi: 10.1002/jat.2550110607.
The joint action of binary mixtures of the osteolathyrogens semicarbazide (SC), beta-aminopropionitrile (beta APN) and penicillamine (PNC) were determined at malformation-inducing concentrations for Xenopus embryos. Tests were static with renewal every 24 h for the 96-h test period. Simultaneous tests on each individual component of the binary mixtures alone gave baseline malformation data (EC50) for joint action analyses. Toxic unit analysis and isobole diagrams were used to determine the type of joint action for 3:1, 1:1 and 1:3 mixtures of each combination. The joint action was concentration additive (strictly additive) for SC with beta APN and response additive (less-than-additive) for SC with PNC and beta APN with PNC. The joint actions were not changed when only osteolathyrogenic lesions, rather than all types of malformations, were considered. The different specific location and character of PNC lesions, as opposed to those for SC and beta APN, may signify a different type of osteolathyrogenic effect for PNC. The mixture testing approach has potential value in determining compounds that act similarly.
在非洲爪蟾胚胎致畸浓度下，测定了骨畸形生成剂氨基脲（SC）、β-氨基丙腈（β-APN）和青霉胺（PNC）二元混合物的联合作用。试验为静态试验，在96小时试验期内每24小时更新一次。对二元混合物的每个单独成分同时进行试验，得出联合作用分析的基线畸形数据（EC50）。使用毒性单位分析和等效线图来确定每种组合的3:1、1:1和1:3混合物的联合作用类型。SC与β-APN的联合作用为浓度相加（严格相加），SC与PNC以及β-APN与PNC的联合作用为反应相加（小于相加）。当仅考虑骨畸形生成性病变而非所有类型的畸形时，联合作用没有改变。与SC和β-APN相比，PNC病变的不同特定位置和特征可能表明PNC具有不同类型的骨畸形生成作用。混合物测试方法在确定作用相似的化合物方面具有潜在价值。
