Smucker R A, Pfister R M
Appl Microbiol. 1975 Sep;30(3):445-9. doi: 10.1128/am.30.3.445-449.1975.
High-efficiency disruption of bacteria can be accomplished in 2 or more min by the new procedure of liquid nitrogen cryo-impacting. Release of the dipicolinic acid-Ca2+ chelate paralleled the breakage of Bacillus megaterium endospores. Lactate dehydrogenase activity was much better in supernates from liquid nitrogen cryo-impacting-broken Escherichia coli cells than in those from sonically treated and broken E. coli cells.
通过液氮冷冻冲击这一新方法，可在2分钟或更短时间内实现对细菌的高效破坏。吡啶二羧酸-Ca2+螯合物的释放与巨大芽孢杆菌芽孢的破裂同步。液氮冷冻冲击破碎的大肠杆菌细胞的上清液中的乳酸脱氢酶活性，比经超声处理破碎的大肠杆菌细胞的上清液中的乳酸脱氢酶活性要好得多。
