通过使用一种新的、灵敏的吡啶二羧酸检测方法研究细菌芽孢萌发过程中吡啶二羧酸钙的释放。
Study of calcium dipicolinate release during bacterial spore germination by using a new, sensitive assay for dipicolinate.
作者信息
Scott I R, Ellar D J
出版信息
J Bacteriol. 1978 Jul;135(1):133-7. doi: 10.1128/jb.135.1.133-137.1978.
Abstract
The release of calcium and dipicolinic acid from spores of Bacillus megaterium KM during L-alanine-induced triggering of germination has been studied using a new, simple, and rapid assay for dipicolinic acid capable of detecting a concentration of 0.5 micron. The release of both calcium and dipicolinate started within seconds of exposure of the spores to L-alanine, thus preceding other measurable changes associated with germination. From the earliest times, the two substances were released in equimolar quantities, although later in germination calcium predominated.
摘要
利用一种能够检测浓度低至0.5微米的新型、简单且快速的吡啶二羧酸检测方法，研究了巨大芽孢杆菌KM菌株的孢子在L-丙氨酸诱导萌发过程中钙和吡啶二羧酸的释放情况。孢子接触L-丙氨酸后数秒内，钙和吡啶二羧酸就开始释放，早于与萌发相关的其他可测量变化。从最初阶段开始，这两种物质以等摩尔量释放，不过在萌发后期钙占主导地位。
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Study of calcium dipicolinate release during bacterial spore germination by using a new, sensitive assay for dipicolinate.通过使用一种新的、灵敏的吡啶二羧酸检测方法研究细菌芽孢萌发过程中吡啶二羧酸钙的释放。
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