基于18S rRNA基因的限制性片段长度多态性（RFLP）技术在媒介和宿主中鉴别小泰累尔梨形虫和环形泰勒虫
Discrimination between Theileria lestoquardi and Theileria annulata in their vectors and hosts by RFLP based on the 18S rRNA gene.
作者信息
Spitalska Eva, Torina Alessandra, Cannella Vincenza, Caracappa Santo, Sparagano Olivier A E
机构信息
School of Agriculture, Food and Rural Development, University of Newcastle, Newcastle upon Tyne, NE1 7RU, UK.
出版信息
Parasitol Res. 2004 Oct;94(4):318-20. doi: 10.1007/s00436-004-1217-2. Epub 2004 Sep 15.
Theileria lestoquardi and T. annulata can occur in similar vectors, and current available probes based on the 18S rRNA gene showed cross-reaction between the two species. However, we developed a species-specific RFLP test based on the MspI restriction enzyme, able to cut amplified products from T. lestoquardi only and to discriminate the two species in both tick and blood samples.
莱氏泰勒虫和环形泰勒虫可出现在相似的媒介中，目前基于18S rRNA基因的现有探针显示这两个物种之间存在交叉反应。然而，我们开发了一种基于MspI限制酶的物种特异性RFLP检测方法，该方法仅能切割莱氏泰勒虫的扩增产物，并能在蜱虫和血液样本中区分这两个物种。
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