Li N
Department of Pathology, Hei Shan Hu Hospital, Beijing.
Zhonghua Zhong Liu Za Zhi. 1993 Jan;15(1):34-6.
Expression of proliferating cell nuclear antigen (PCNA/Cyclin) in 73 cases of gastric carcinomas and its relationship to prognosis were semiquantitatively analysed by ABC immunoperoxidase method and its clinical application was explored. PCNA staining located in the nuclei of tumor cells. A semiquantitative PCNA grading showed a significant negative correlation with survival of the gastric carcinomas cases (P < 0.01). Survival analysis also presented that the prognosis of patients with intensive expression of PCNA was poor. Generally speaking, the expression of PCNA showed a significant positive correlation with the differentiation degree of the tumor and nodal metastasis (P < 0.05). However, among these cases, the poorly differentiated adenocarcinomas with a weak grade of PCNA expression generally considered as of a high malignant degree and poor prognosis, survived longer. The facts indicated that the in situ detection of PCNA in gastric carcinoma is more objective and reliable in assessing a proper prognosis than that made on the basis of traditional morphological differentiation grading.
采用ABC免疫过氧化物酶法对73例胃癌组织中增殖细胞核抗原（PCNA/细胞周期蛋白）的表达及其与预后的关系进行半定量分析，并探讨其临床应用价值。PCNA染色位于肿瘤细胞核内。PCNA半定量分级显示与胃癌患者的生存率呈显著负相关（P<0.01）。生存分析还表明，PCNA表达强的患者预后较差。一般来说，PCNA的表达与肿瘤的分化程度和淋巴结转移呈显著正相关（P<0.05）。然而，在这些病例中，PCNA表达较弱的低分化腺癌通常被认为恶性程度高、预后差，但生存期较长。这些事实表明，胃癌中PCNA的原位检测在评估预后方面比基于传统形态学分化分级更客观、可靠。
