利用线粒体DNA对疟疾媒介咸水按蚊进行种群分化研究
Population differentiation of the malaria vector Anopheles aquasalis using mitochondrial DNA.
作者信息
Conn J, Cockburn A F, Mitchell S E
机构信息
Florida Medical Entomology Laboratory, Vero Beach.
出版信息
J Hered. 1993 Jul-Aug;84(4):248-53. doi: 10.1093/oxfordjournals.jhered.a111334.
Populations of the malaria vector Anopheles aquasalis from Venezuela, Trinidad, and Brazil were analyzed using restriction enzyme digestion of mitochondrial DNA (mtDNA). The five enzymes surveyed yielded 12 mtDNA haplotypes. Three restriction endonuclease profiles (Bcl I, Eco RI, and Hind III) differentiated the Venezuelan and Trinidadian A. aquasalis from two Brazilian populations. Estimates of mtDNA sequence divergence between the Venezuelan/Trinidadian populations and each Brazilian population (0.023-0.032), as well as divergence between the two Brazilian populations (0.030), were within the range of interspecific distances calculated for members of anopheline species complexes. Ribosomal DNA (rDNA) restriction profiles of the A. aquasalis populations examined were identical.
利用线粒体DNA（mtDNA）的限制性酶切消化技术，对来自委内瑞拉、特立尼达和巴西的疟疾媒介——咸水按蚊种群进行了分析。所检测的5种酶产生了12种mtDNA单倍型。三种限制性内切酶图谱（Bcl I、Eco RI和Hind III）区分了来自委内瑞拉和特立尼达的咸水按蚊与两个巴西种群。委内瑞拉/特立尼达种群与每个巴西种群之间的mtDNA序列差异估计值（0.023 - 0.032），以及两个巴西种群之间的差异（0.030），均在按蚊物种复合体成员种间距离的计算范围内。所检测的咸水按蚊种群的核糖体DNA（rDNA）限制性图谱是相同的。
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