Angêlla Aline F, Gil Luis H S, Silva Luis H P da, Ribolla Paulo E M
Departamento de Parasitologia, Instituto de Biociências, Universidade Estadual Paulista, 18618-000 Botucatu, SP, Brasil.
Mem Inst Oswaldo Cruz. 2007 Dec;102(8):953-8. doi: 10.1590/s0074-02762007000800010.
Anopheles darlingi is the most important Brazilian malaria vector, with a widespread distribution in the Amazon forest. Effective strategies for vector control could be better developed through knowledge of its genetic structure and gene flow among populations, to assess the vector diversity and competence in transmitting Plasmodium. The aim of this study was to assess the genetic diversity of An. darlingi collected at four locations in Porto Velho, by sequencing a fragment of the ND4 mitochondrial gene. From 218 individual mosquitoes, we obtained 20 different haplotypes with a diversity index of 0.756, equivalent to that found in other neotropical anophelines. The analysis did not demonstrate significant population structure. However, haplotype diversity within some populations seems to be over-represented, suggesting the presence of sub-populations, but the presence of highly represented haplotypes complicates this analysis. There was no clear correlation among genetic and geographical distance and there were differences in relation to seasonality, which is important for malarial epidemiology.
达林按蚊是巴西最重要的疟疾传播媒介，在亚马逊森林广泛分布。通过了解其遗传结构和种群间的基因流动，能够更好地制定有效的媒介控制策略，以评估媒介在传播疟原虫方面的多样性和能力。本研究的目的是通过对ND4线粒体基因片段进行测序，评估在波多韦柳四个地点采集的达林按蚊的遗传多样性。从218只个体蚊子中，我们获得了20种不同的单倍型，多样性指数为0.756，与在其他新热带地区按蚊中发现的指数相当。分析未显示出显著的种群结构。然而，一些种群内的单倍型多样性似乎被过度代表，这表明存在亚种群，但高度代表的单倍型的存在使这一分析变得复杂。遗传距离和地理距离之间没有明显的相关性，并且在季节性方面存在差异，这对疟疾流行病学很重要。
