Coleman N, Greenfield I M, Hare J, Kruger-Gray H, Chain B M, Stanley M A
Department of Pathology, University of Cambridge, United Kingdom.
Am J Pathol. 1993 Aug;143(2):355-67.
We have investigated the expression of intercellular adhesion molecule-1 (ICAM-1) in squamous neoplasia of the cervix and have noted a significant induction of the molecule in high-grade intra-epithelial lesions. Using monolayer and organotypic in vitro tissue culture systems, we have shown that there is no constitutive ICAM-1 expression on cervical keratinocytes immortalized but not transformed by human papillomavirus type 16, whereas two human papillomaviruses type 16 containing and fully transformed cervical keratinocyte lines do constitutively express the molecule. All cell types, including human papillomavirus-negative normal cervical keratinocytes, can be induced to up-regulate their expression of ICAM-1 by pro-inflammatory cytokines such as interferon-gamma. In addition, we have used an in vitro adhesion assay to show that ICAM-1:lymphocyte function antigen-1 interaction is functionally important in lymphocyte binding to cervical keratinocytes, suggesting a role for ICAM-1 in retaining and enabling functional activity of lymphocytes in the cervix in intraepithelial neoplasia.
我们研究了细胞间黏附分子-1（ICAM-1）在宫颈鳞状上皮瘤变中的表达情况，发现在高级别上皮内病变中该分子有显著诱导表达。利用单层和器官型体外组织培养系统，我们发现，经人乳头瘤病毒16型永生化但未转化的宫颈角质形成细胞不组成性表达ICAM-1，而两条含有并完全转化人乳头瘤病毒16型的宫颈角质形成细胞系则组成性表达该分子。所有细胞类型，包括人乳头瘤病毒阴性的正常宫颈角质形成细胞，均可被促炎细胞因子如干扰素-γ诱导上调其ICAM-1表达。此外，我们利用体外黏附试验表明，ICAM-1与淋巴细胞功能抗原-1的相互作用在淋巴细胞与宫颈角质形成细胞的结合中具有重要功能，提示ICAM-1在宫颈上皮内瘤变中对于保留淋巴细胞并使其发挥功能活性具有作用。
