Chang H, Wu G J, Wang S M, Hung C R
Department of Emergency Medicine, National Taiwan University Hospital, Taipei, R.O.C.
J Formos Med Assoc. 1993 Mar;92(3):207-12.
In this study, aortic strips taken from mongrel dogs were used as experimental material. After preparation, the aortic strips were incubated in a designed control medium or an experimental medium (adding thrombin or epinephrine to the control medium). The pH value of each incubation medium mentioned above was adjusted with HCl or NaOH to a level of 8.0, 7.4, 7.0 and 6.6, respectively, in order to investigate the influence of medium acidity on the production of endothelin-1 from endothelial cells using aortic strips. After a designated time duration (one hour, three hours, six hours and 12 hours, respectively), the incubation media were collected for assay of endothelin-1. The results demonstrated that the endothelin-1 level of the incubation medium was elevated when: 1) the incubation time was lengthened; 2) the incubation medium was more acidic; 3) the aortic strips were incubated with a stimulant, such as thrombin and epinephrine; and 4) the endothelial cells on the aortic strips were intact. This indicates that the secretion of endothelin-1 is: 1) time-dependent, 2) pH-dependent, 3) stimulant-dependent and 4) endothelium-dependent.
在本研究中，选用杂种犬的主动脉条作为实验材料。制备后，将主动脉条置于设计好的对照培养基或实验培养基（在对照培养基中添加凝血酶或肾上腺素）中孵育。上述每种孵育培养基的pH值分别用HCl或NaOH调节至8.0、7.4、7.0和6.6，以研究培养基酸度对利用主动脉条的内皮细胞产生内皮素-1的影响。在指定的时间段（分别为1小时、3小时、6小时和12小时）后，收集孵育培养基用于检测内皮素-1。结果表明，当以下情况时，孵育培养基中的内皮素-1水平升高：1）孵育时间延长；2）孵育培养基酸性更强；3）主动脉条与刺激物（如凝血酶和肾上腺素）一起孵育；4）主动脉条上的内皮细胞完整。这表明内皮素-1的分泌：1）依赖时间，2）依赖pH值，3）依赖刺激物，4）依赖内皮。
