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[尿路致病性大肠杆菌中P菌毛的检测方法]

[Methods of detection of P-fimbria in uropathogenic E. coli].

作者信息

Bartková G, Ciznár I

机构信息

Ustav preventívnej a klinickej medicíny, Bratislava.

出版信息

Cesk Epidemiol Mikrobiol Imunol. 1993 Jun;42(2):54-8.

PMID:8102326
Abstract

We characterized the surface properties of E. coli strains isolated from urine of children with urinary tract infections. The PF test and haemagglutination reaction were used for detection of P-fimbriae. P-fimbriae were detected in 30-54% of the pyelonephritic strains. All these strains expressed P-fimbriae in vitro. Pap genes were determined by a DNA probe in 85% of the strains isolated from pyelonephritis. These strains showed diffuse and localized adherence to tissue culture cells without any association to the expression of P-fimbriae on agar medium.

摘要

我们对从患有尿路感染的儿童尿液中分离出的大肠杆菌菌株的表面特性进行了表征。采用PF试验和血凝反应检测P菌毛。在30%-54%的肾盂肾炎菌株中检测到了P菌毛。所有这些菌株在体外均表达P菌毛。通过DNA探针在85%从肾盂肾炎分离出的菌株中检测到了Pap基因。这些菌株对组织培养细胞表现出弥漫性和局限性黏附,且与琼脂培养基上P菌毛的表达无任何关联。

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Cesk Epidemiol Mikrobiol Imunol. 1993 Jun;42(2):54-8.
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