Cejková J, Potuzník V
Ustav lékarské mikrobiologie FDL KU, Praha.
Cesk Epidemiol Mikrobiol Imunol. 1991 Mar;40(2):65-8.
The PF test of the Finnish firm Orion is used for detection of P fimbriae in E. coli strains. Its principle involves the establishment of a chemically defined receptor for these fimbriae on latex particles which can then be agglutinated by the appropriate strains. By means of this test the authors examined 453 uropathogenic E. coli strains. P fimbriae were detected in 121 strains, i.e. in 26.7%. Twenty-five strains agglutinated not only latex particles coated with receptor but also non-coated control particles. The test was thus falsely positive in 5.5% of the cases. Fifty-five strains, where by means of the PF test fimbriae were detected, were examined by the method of mannoresistent haemagglutination of human erythrocytes on a slide. The erythrocytes agglutinated in the presence of d-mannose of all 55 strains, and thus the results agreed in 100%. This suggests that P fimbriae can be detected by the haemagglutination method, but it must be taken into account that this method can, contrary to the PF test, detect also other adhesins. The PF test is very quick, simple and its principle ensures a high reliability.
芬兰奥立安公司的PF试验用于检测大肠杆菌菌株中的P菌毛。其原理是在乳胶颗粒上建立这些菌毛的化学定义受体，然后可被相应菌株凝集。通过该试验，作者检测了453株尿路致病性大肠杆菌菌株。在121株菌株中检测到P菌毛，即占26.7%。25株菌株不仅使包被有受体的乳胶颗粒凝集，还使未包被的对照颗粒凝集。因此，该试验在5.5%的病例中出现假阳性。对55株通过PF试验检测到菌毛的菌株，采用玻片上人红细胞甘露糖抗性血凝试验进行检测。所有55株菌株在d-甘露糖存在下红细胞均发生凝集，结果100%一致。这表明P菌毛可用血凝试验检测，但必须考虑到，与PF试验不同，该方法也能检测其他黏附素。PF试验非常快速、简单，其原理确保了高可靠性。
