PTU treatment-induced hypothyroidism is associated with a significant decrease in the chick heart rate. 2. Hypothyroidism produces a slow onset of bradycardia, indicating a late effect of thyroid hormone decrease. 3. After 15 days treatment, an increase of action potential duration, similar to that reported for several mammal species, has been found. 4. Action potential duration is frequency and temperature dependent, but it has been found to be significantly different in the euthyroid and hypothyroid chicks.