马疱疹病毒2型（EHV-2）和单纯疱疹病毒1型（HSV-1）基因与马疱疹病毒1型（EHV-1）的交叉杂交。

Cross-hybridization of equid herpesvirus-2 (EHV-2) and herpes simplex virus-1 (HSV-1) genes to equid herpesvirus-1 (EHV-1).

作者信息

Purewal A S, Smallwood A V, Allsopp R, Welch H M, Edington N

机构信息

Department of Veterinary Pathology, Royal Veterinary College, London, UK.

出版信息

Vet Microbiol. 1993 May;35(1-2):1-10. doi: 10.1016/0378-1135(93)90111-j.

DOI:10.1016/0378-1135(93)90111-j

PMID:8103247

Abstract

In contrast to previous findings, the Ab4 isolate of equid herpesvirus-1 (EHV-1) was shown to share homology with the G9 isolate of equid herpesvirus-2 (EHV-2). Using Southern blotting and stringent hybridization conditions, a significant proportion of this cross-hybridization was identified by the immediate-early gene-3 (IE-3) probe from herpes simplex virus-1 (HSV-1). The HSV-1 UL48 gene probe (encoding the IE gene transactivating protein VmW65, which is also known as alpha-TIF or VP16) was used to identify and isolate its counterpart in EHV-1. The relevance of shared homology to transactivation is being investigated.

摘要

与之前的研究结果相反，马疱疹病毒1型（EHV-1）的Ab4分离株被证明与马疱疹病毒2型（EHV-2）的G9分离株具有同源性。使用Southern印迹法和严格的杂交条件，单纯疱疹病毒1型（HSV-1）的立即早期基因3（IE-3）探针鉴定出了相当一部分这种交叉杂交。HSV-1 UL48基因探针（编码IE基因反式激活蛋白VmW65，也称为α-TIF或VP16）被用于鉴定和分离其在EHV-1中的对应物。正在研究共享同源性与反式激活的相关性。