构建细胞遗传学特征明确的猪体细胞杂交板及其作为定位工具的应用。
Construction of a cytogenetically characterized porcine somatic cell hybrid panel and its use as a mapping tool.
作者信息
Zijlstra C, Bosma A A, de Haan N A, Mellink C
机构信息
Department of Cell Biology and Histology, Faculty of Veterinary Medicine, Utrecht University, PO Box 80.157, 3508 TD Utrecht, The Netherlands.
出版信息
Mamm Genome. 1996 Apr;7(4):280-4. doi: 10.1007/s003359900083.
A new panel of cytogenetically characterized pig-rodent somatic cell hybrids was constructed and tested for twelve microsatellite markers with PCR. Cytogenetic characterization of hybrids was accomplished by fluorescence painting and GTG-banding of metaphase chromosomes. The panel consists of 15 independent pig-hamster and 6 independent pig-mouse cell lines. In the panel, all pig autosomes and the X Chromosome (Chr) are represented, and it is informative for all chromosome pairs except 2-14, 2-15, 3-9, 14-15, 14-16, and 16-17. The microsatellites tested were S0022, S0023, S0084, S0098, S0112, S0113, S0114, S0115, S0117, S0118, S0119, and S0120. The PCR results obtained in the 21 hybrids were compared with the cytogenetic data and analyzed for concordancy and correlation. Eight microsatellites could be assigned to specific pig chromosomes, confirming seven assignments based on linkage analysis.
构建了一组新的经细胞遗传学特征鉴定的猪-啮齿动物体细胞杂种，并通过聚合酶链反应（PCR）对12个微卫星标记进行了检测。杂种的细胞遗传学特征鉴定通过中期染色体的荧光原位杂交和GTG显带技术完成。该组包括15个独立的猪-仓鼠和6个独立的猪-小鼠细胞系。在该组中，所有猪常染色体和X染色体（Chr）均有代表，除2-14、2-15、3-9、14-15、14-16和16-17染色体对外，对所有染色体对均有信息价值。所检测的微卫星标记为S0022、S0023、S0084、S0098、S0112、S0113、S0114、S0115、S0117、S0118、S0119和S0120。将在21个杂种中获得的PCR结果与细胞遗传学数据进行比较，并分析其一致性和相关性。8个微卫星标记可定位到特定的猪染色体上，基于连锁分析证实了7个定位结果。
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