Chen S L, Han C P, Tsao Y P, Lee J W, Yin C S
Department of Microbiology and Immunology, National Defense Medical Center, Taipei, Taiwan, Republic of China.
Cancer. 1993 Sep 15;72(6):1939-45. doi: 10.1002/1097-0142(19930915)72:6<1939::aid-cncr2820720624>3.0.co;2-2.
Although human papillomaviruses (HPV) are associated with cervical cancer, it has yet to be determined if specific HPV types have clinical or prognostic significance.
Identification and typing of HPV were done by polymerase chain reaction (PCR) and restriction fragment length polymorphism.
Of the 43 cases of cervical cancer, 31 (72%) were HPV positive. The results of HPV typing in 40 cases of squamous cell carcinoma of the cervix revealed the presence of HPV-16 in 20 cases (50%), HPV-18 in 2 cases, HPV-11 in 1 case, HPV-33 in 1 case, HPV-52 in 1 case, HPV-58 in 1 case, and unidentified HPV types in 5 cases. Neither HPV-31 nor HPV-42 were present in our study. One case of squamous cell carcinoma had HPV-11 integration. Chi-square analysis revealed significant correlation between HPV genotypes and squamous cell patterns, no significant correlation between HPV genotypes and clinical stages, and cell differentiation of squamous cell carcinoma of cervix.
These findings may contribute to understanding the role of HPV in cancer and the value of typing as a prognostic indicator.
尽管人乳头瘤病毒（HPV）与宫颈癌相关，但特定HPV类型是否具有临床或预后意义尚未确定。
通过聚合酶链反应（PCR）和限制性片段长度多态性进行HPV的鉴定和分型。
43例宫颈癌患者中，31例（72%）HPV阳性。40例宫颈鳞状细胞癌的HPV分型结果显示，20例（50%）存在HPV-16，2例存在HPV-18，1例存在HPV-11，1例存在HPV-33，1例存在HPV-52，1例存在HPV-58，5例为未鉴定的HPV类型。本研究中未发现HPV-31和HPV-42。1例鳞状细胞癌存在HPV-11整合。卡方分析显示，HPV基因型与鳞状细胞模式之间存在显著相关性，HPV基因型与临床分期以及宫颈鳞状细胞癌的细胞分化之间无显著相关性。
这些发现可能有助于理解HPV在癌症中的作用以及分型作为预后指标的价值。
