宫颈癌中人乳头瘤病毒16型和18型的流行情况：斑点杂交、Southern印迹杂交及聚合酶链反应研究
Prevalence of human papillomavirus types 16 and 18 in cervical carcinomas: a study by dot and Southern blot hybridization and the polymerase chain reaction.
作者信息
Low S H, Thong T W, Ho T H, Lee Y S, Morita T, Singh M, Yap E H, Chan Y C
机构信息
Department of Microbiology, National University of Singapore.
出版信息
Jpn J Cancer Res. 1990 Nov;81(11):1118-23. doi: 10.1111/j.1349-7006.1990.tb02522.x.
Abstract
Histologically classified biopsies from 83 women with invasive cervical carcinoma were analyzed by dot blot hybridization for human papillomavirus (HPV) types 16 and 18 infection. Sixty of the 83 (72.3%) were found to contain HPV DNA, of which 43 (51.8%) contained HPV 16 DNA, 12 (14.5%) contained HPV 18 DNA and 5 (6.0%) contained both HPV 16 and 18 DNAs. Southern blot analysis on 65 specimens gave similar results. Of 23 specimens negative by dot blot, 21 were tested by the polymerase chain reaction. Seventeen of the 21 were positive for HPV DNA, of which 13 contained HPV 16 DNA and 4 contained both HPV 16 and 18 DNAs. In all, 95.1% (77/81) were positive for HPV 16 and/or 18 DNA sequences.
摘要
对83例浸润性宫颈癌女性的组织学分类活检标本进行斑点杂交分析，以检测人乳头瘤病毒（HPV）16型和18型感染情况。83例中有60例（72.3%）被发现含有HPV DNA，其中43例（51.8%）含有HPV 16 DNA，12例（14.5%）含有HPV 18 DNA，5例（6.0%）同时含有HPV 16和18 DNA。对65份标本进行的Southern印迹分析得到了相似结果。在斑点杂交检测为阴性的23份标本中，有21份进行了聚合酶链反应检测。21份中有17份HPV DNA呈阳性，其中13份含有HPV 16 DNA，4份同时含有HPV 16和18 DNA。总体而言，95.1%（77/81）的标本HPV 16和/或18 DNA序列呈阳性。
相似文献
1
Prevalence of human papillomavirus types 16 and 18 in cervical carcinomas: a study by dot and Southern blot hybridization and the polymerase chain reaction.宫颈癌中人乳头瘤病毒16型和18型的流行情况：斑点杂交、Southern印迹杂交及聚合酶链反应研究
Jpn J Cancer Res. 1990 Nov;81(11):1118-23. doi: 10.1111/j.1349-7006.1990.tb02522.x.
2
Alterations of the p53 gene in human primary cervical carcinoma with and without human papillomavirus infection.人乳头瘤病毒感染阳性和阴性的原发性宫颈癌中p53基因的改变
Cancer Res. 1992 Oct 1;52(19):5323-8.
3
Detection of human papillomavirus (HPV) type 16 and 52b in cervical cancer tissues by Southern blot hybridization and polymerase chain reaction (PCR).通过Southern印迹杂交和聚合酶链反应（PCR）检测宫颈癌组织中的16型和52b型人乳头瘤病毒（HPV）
Virus Genes. 1990 Dec;4(4):313-23. doi: 10.1007/BF00570026.
4
DNA sequences of human papillomavirus types 11, 16 or 18 in invasive cervical carcinoma of Western Australian women.西澳大利亚女性浸润性宫颈癌中11、16或18型人乳头瘤病毒的DNA序列
Immunol Cell Biol. 1987 Feb;65 ( Pt 1):77-84. doi: 10.1038/icb.1987.9.
5
A high frequency of human papillomavirus DNA sequences in cervical carcinomas of Indian women as revealed by Southern blot hybridization and polymerase chain reaction.通过Southern印迹杂交和聚合酶链反应揭示，印度女性宫颈癌中人乳头瘤病毒DNA序列的高频率存在。
J Med Virol. 1992 Apr;36(4):239-45. doi: 10.1002/jmv.1890360402.
6
Correlation of histologic types of carcinoma of the uterine cervix and human papillomavirus and herpes simplex virus type 2 DNA sequences in the uterine cervical biopsies.子宫颈活检组织中子宫颈癌组织学类型与人乳头瘤病毒及2型单纯疱疹病毒DNA序列的相关性
Arch Virol. 1988;102(1-2):141-6. doi: 10.1007/BF01315571.
7
Human papillomavirus DNA sequences in adenocarcinoma of the uterine cervix in Indian women.印度女性子宫颈腺癌中的人乳头瘤病毒DNA序列
Cancer. 1993 Jul 1;72(1):147-53. doi: 10.1002/1097-0142(19930701)72:1<147::aid-cncr2820720128>3.0.co;2-7.
8
Diagnostic sensitivity of polymerase chain reaction and Southern blot hybridization for the detection of human papillomavirus DNA in biopsy specimens from cervical lesions.聚合酶链反应和Southern印迹杂交法检测宫颈病变活检标本中人乳头瘤病毒DNA的诊断敏感性
Am J Clin Pathol. 1991 May;95(5):638-46. doi: 10.1093/ajcp/95.5.638.
9
Presence of human papillomavirus type-16 and type-18 DNA sequences and their expression in cervical cancers and cell lines from Japanese patients.
Int J Cancer. 1986 Apr 15;37(4):499-503. doi: 10.1002/ijc.2910370405.
10
Human papillomavirus associated with adenocarcinoma and adenosquamous carcinoma of the cervix: analysis by in situ hybridization.人乳头瘤病毒与宫颈腺癌及腺鳞癌的关系：原位杂交分析
Gynecol Oncol. 1989 Dec;35(3):345-8. doi: 10.1016/0090-8258(89)90076-0.
引用本文的文献
1
Comparison of AmpFire and MY09/11 assays for HPV genotyping in anogenital specimen of Rwandan men who have sex with men.AmpFire与MY09/11检测法在卢旺达男男性行为者肛门生殖器标本中进行人乳头瘤病毒基因分型的比较
Heliyon. 2023 May 1;9(5):e16016. doi: 10.1016/j.heliyon.2023.e16016. eCollection 2023 May.
2
Association between human Papillomavirus and colorectal adenocarcinoma and its influence on tumor staging and degree of cell differentiation.人乳头瘤病毒与结直肠癌的关联及其对肿瘤分期和细胞分化程度的影响。
Arq Bras Cir Dig. 2014 Jul-Sep;27(3):172-6. doi: 10.1590/s0102-67202014000300003.
3
High-resolution melting molecular signatures for rapid identification of human papillomavirus genotypes.高分辨率熔解分子特征用于快速鉴定人乳头瘤病毒基因型。
PLoS One. 2012;7(8):e42051. doi: 10.1371/journal.pone.0042051. Epub 2012 Aug 20.
4
Comparison between the Hybrid Capture II Test and an SPF1/GP6+ PCR-based assay for detection of human papillomavirus DNA in cervical swab samples.杂交捕获二代检测法与基于SPF1/GP6+聚合酶链反应的检测法在宫颈拭子样本中检测人乳头瘤病毒DNA的比较。
J Clin Microbiol. 2006 May;44(5):1733-9. doi: 10.1128/JCM.44.5.1733-1739.2006.
5
Development and clinical evaluation of a highly sensitive PCR-reverse hybridization line probe assay for detection and identification of anogenital human papillomavirus.用于检测和鉴定肛门生殖器人乳头瘤病毒的高灵敏度聚合酶链反应-反向杂交线性探针检测法的开发与临床评估
J Clin Microbiol. 1999 Aug;37(8):2508-17. doi: 10.1128/JCM.37.8.2508-2517.1999.
6
Serum antibodies to Trichomonas vaginalis in invasive cervical cancer patients.浸润性宫颈癌患者血清中抗阴道毛滴虫抗体
Genitourin Med. 1995 Dec;71(6):402-4. doi: 10.1136/sti.71.6.402.
7
Comparison of Virapap filter hybridization with polymerase chain reaction and Southern blot hybridization methods for detection of human papillomavirus in tonsillar and pharyngeal cancers.Virapap滤膜杂交法与聚合酶链反应及Southern印迹杂交法在检测扁桃体癌和咽癌中人乳头瘤病毒的比较
Eur Arch Otorhinolaryngol. 1993;250(2):115-9. doi: 10.1007/BF00179310.
本文引用的文献
1
Presence of human papillomavirus in genital tumors.生殖器肿瘤中人类乳头瘤病毒的存在。
J Invest Dermatol. 1984 Jul;83(1 Suppl):26s-28s. doi: 10.1111/1523-1747.ep12281143.
2
A new type of papillomavirus DNA, its presence in genital cancer biopsies and in cell lines derived from cervical cancer.一种新型乳头瘤病毒DNA，其在生殖器癌活检组织及源自宫颈癌的细胞系中的存在情况。
EMBO J. 1984 May;3(5):1151-7. doi: 10.1002/j.1460-2075.1984.tb01944.x.
3
Current concepts of human papillomavirus infections in the genital tract and their relationship to intraepithelial neoplasia and squamous cell carcinoma.生殖道中人乳头瘤病毒感染的当前概念及其与上皮内瘤变和鳞状细胞癌的关系。
Obstet Gynecol Surv. 1984 May;39(5):252-65. doi: 10.1097/00006254-198405000-00002.
4
A technique for radiolabeling DNA restriction endonuclease fragments to high specific activity.一种将DNA限制性内切酶片段放射性标记至高比活度的技术。
Anal Biochem. 1983 Jul 1;132(1):6-13. doi: 10.1016/0003-2697(83)90418-9.
5
A papillomavirus DNA from a cervical carcinoma and its prevalence in cancer biopsy samples from different geographic regions.一种来自宫颈癌的乳头瘤病毒DNA及其在不同地理区域癌症活检样本中的流行情况。
Proc Natl Acad Sci U S A. 1983 Jun;80(12):3812-5. doi: 10.1073/pnas.80.12.3812.
6
Enzymatic gene amplification: qualitative and quantitative methods for detecting proviral DNA amplified in vitro.酶促基因扩增：检测体外扩增的前病毒DNA的定性和定量方法。
J Infect Dis. 1988 Dec;158(6):1158-69. doi: 10.1093/infdis/158.6.1158.
7
DNA sequences of human papillomavirus types 11, 16 or 18 in invasive cervical carcinoma of Western Australian women.西澳大利亚女性浸润性宫颈癌中11、16或18型人乳头瘤病毒的DNA序列
Immunol Cell Biol. 1987 Feb;65 ( Pt 1):77-84. doi: 10.1038/icb.1987.9.
8
Human papillomavirus types 16 and 18 sequences in early cervical neoplasia.早期宫颈肿瘤中16型和18型人乳头瘤病毒序列
Virology. 1986 Nov;155(1):13-8. doi: 10.1016/0042-6822(86)90163-7.
9
Detection of human papillomavirus DNA in genital warts, cervical dysplasias and neoplasias.尖锐湿疣、宫颈发育异常及肿瘤中人类乳头瘤病毒DNA的检测
Intervirology. 1986;25(3):151-7. doi: 10.1159/000149669.
10
Human papillomavirus type 16 DNA in genital tumours: a pathological and molecular analysis.生殖器肿瘤中的人乳头瘤病毒16型DNA：病理与分子分析
J Gen Virol. 1986 Mar;67 ( Pt 3):583-9. doi: 10.1099/0022-1317-67-3-583.
Zotero 插件