Tsao Y P, Chu T Y, Chen T M, Yang Y F, Chen S L
Department of Microbiology and Immunology, National Defense Medical Center, Taipei, Taiwan, Republic of China.
Arch Virol. 1994;138(1-2):177-85. doi: 10.1007/BF01310049.
The E5a gene of HPV-11 expressed in NIH 3T3 cells led to tumorigenesis in nude mice; whereas when expressed in keratinocytes, E5a induced anchorage independent growth, but was nontumorigenic in nude mice. The E7 gene of HPV-11 expressed NIH 3T3 cells led to tumors in nude mice and morphological transformation, but not anchorage independent growth. Keratinocytes expressing the E7 gene induced colony formation in soft agarose, but not tumorigenesis in nude mice. Comparison of transforming activities of HPV-11 E5a and E7 genes of HPV-11 showed that the efficiency of cell transformation by E7 was weaker than that of E5a.
在NIH 3T3细胞中表达的HPV - 11的E5a基因可导致裸鼠发生肿瘤；而当在角质形成细胞中表达时，E5a诱导非贴壁依赖性生长，但在裸鼠中不具有致瘤性。在NIH 3T3细胞中表达的HPV - 11的E7基因可导致裸鼠发生肿瘤并出现形态学转化，但不具有非贴壁依赖性生长。表达E7基因的角质形成细胞可在软琼脂糖中诱导集落形成，但在裸鼠中不具有致瘤性。HPV - 11的E5a和E7基因转化活性的比较表明，E7对细胞的转化效率低于E5a。
