Enyaru J C, Allingham R, Bromidge T, Kanmogne G D, Carasco J F
Uganda Trypanosomiasis Research Organization, Tororo.
Acta Trop. 1993 Jun;54(1):31-9. doi: 10.1016/0001-706x(93)90066-k.
Fifty-two samples of blood were taken from sleeping sickness patients in north-west Uganda. All samples failed to infect immunosuppressed mice. Ten cryopreserved blood samples were fed to laboratory bred Glossina morsitans morsitans; eight flies developed midgut infections from which procyclic cultures were established in vitro. Isoenzyme electrophoretic analysis of 9 enzymes revealed that 7 of the 8 trypanosome isolates had a combination of enzyme patterns already described for Trypanosoma brucei gambiense. The eighth isolate had a different aspartate aminotransferase polymorphism which placed it in a new zymodeme. Analysis of polymorphisms in genes for 3 variant surface glycoproteins (VSGs) confirmed that the 8 Ugandan trypanosome isolates were T.b.gambiense and revealed further heterogeneity. The VSG 117 gene was present in all the isolates in a pattern of fragments (equivalent to AnTat 1.8) characteristic for T.b.gambiense. For two other VSG genes characteristic of T.b.gambiense, the LiTat 1.3 gene was present in all the isolates, while the AnTat 11.17 gene was present in only 2 of the 8 isolates.
从乌干达西北部的昏睡病患者身上采集了52份血样。所有样本均未能感染免疫抑制小鼠。将10份冷冻保存的血样喂给实验室饲养的冈比亚采采蝇；8只苍蝇出现中肠感染，并在体外建立了前循环培养物。对9种酶进行的同工酶电泳分析表明，8株锥虫分离株中有7株具有已描述的布氏冈比亚锥虫的酶谱组合。第八株分离株具有不同的天冬氨酸转氨酶多态性，将其归入一个新的酶型。对3种可变表面糖蛋白（VSG）基因多态性的分析证实，这8株乌干达锥虫分离株为布氏冈比亚锥虫，并显示出进一步的异质性。VSG 117基因以片段形式存在于所有分离株中（相当于AnTat 1.8），这是布氏冈比亚锥虫的特征模式。对于布氏冈比亚锥虫的另外两个特征性VSG基因，LiTat 1.3基因存在于所有分离株中，而AnTat 11.17基因仅存在于8株分离株中的2株中。
