Bae C D, Park S E, Seong Y S, Kimm S W, Park J B, Park J B
Department of Biochemistry, Seoul National University College of Medicine, Korea.
J Korean Med Sci. 1993 Apr;8(2):153-9. doi: 10.3346/jkms.1993.8.2.153.
c-erbB-2 oncogene encodes a growth factor receptor whose amino acid sequence has extensive homology with human epidermal growth factor receptor. It is frequently overexpressed in human breast, ovary, lung, and stomach cancers, where its overexpression is related significantly to the prognosis. Tl investigate the possible role of c-erbB-2 oncogene in the oncogenesis of stomach cancer, we examined the genetic alterations of c-erbB-2 oncogene in 4 stomach cancer cell lines, SNU-1, SNU-5, SNU-16 and KATO III. There were no differences in c-erbB-2 mRNA level as well as c-erbB-2 gene copy number among them. But gp185-erbB-2, c-erbB-2 gene product, was increased from 2- to 4-fold in SNU-1 and SNU-5 cells, compared with that in SNU-16 or KATO III cells. Our results suggest that post-transcriptional regulation of gp185erbB-2 expression may underlie gp185erbB-2 overexpression in cancer cells.
c-erbB-2癌基因编码一种生长因子受体，其氨基酸序列与人类表皮生长因子受体具有广泛的同源性。它在人类乳腺癌、卵巢癌、肺癌和胃癌中经常过度表达，其过度表达与预后显著相关。为了研究c-erbB-2癌基因在胃癌发生中的可能作用，我们检测了4种胃癌细胞系SNU-1、SNU-5、SNU-16和KATO III中c-erbB-2癌基因的基因改变。它们之间的c-erbB-2 mRNA水平以及c-erbB-2基因拷贝数没有差异。但是，与SNU-16或KATO III细胞相比，c-erbB-2基因产物gp185-erbB-2在SNU-1和SNU-5细胞中增加了2至4倍。我们的结果表明，gp185erbB-2表达的转录后调控可能是癌细胞中gp185erbB-2过度表达的基础。
