Bunt A H, Hendrickson A E, Lund J S, Lund R D, Fuchs A F
J Comp Neurol. 1975 Dec 1;164(3):265-85. doi: 10.1002/cne.901640302.
This paper presents evidence on the retinal distribution and central projections of retinal ganglion cells of various cell body sizes in the adult macaque monkey. The ganglion cell sizes have been determined by computer assisted measurement of camera lucida drawings at various eccentricities of both flat mounted and sectioned retinae. The pattern of projections of individual ganglion cells to the dorsal lateral geniculate nucleus and superior colliculus has been studied using retrograde axonal transport of horseradish peroxidase. Following peroxidase injections into the parvocellular laminae of the geniculate, virtually every ganglion cell was labeled within a circumscribed zone of the retina known to project to the region of the geniculate immediately surrounding the injection needle tip. After magnocellular injections, only the largest cells of the peripheral retina and approximately 26% of the ganglion cells of the parafovea were labeled. Peroxidase injections into the superior colliculus produced labeling of scattered ganglion cells of all sizes in the retina, although no labeled cells were found within the centralmost 10 degrees eccentricity. From these observations it is concluded that all ganglion cells of the macaque retina project to the parvocellular layers of the dorsal lateral geniculate, but that only the largest ganglion cells of the more peripheral retina and not all cells of the parafovea project to the magnocellular laminae. In contrast, only scattered ganglion cells, although these are of all sizes, appear to project to the superior colliculus. Two major problems with the peroxidase tracing technique are described: 1. The extent of stainable peroxidase activity around the injection site appears to be larger than the area of injected tracer actually available for uptake by axons to produce labeled cells. 2. Cut or damaged axons appear to incorporate peroxidase sufficiently to produce labeling of the cell body.
本文提供了关于成年猕猴中不同细胞体大小的视网膜神经节细胞的视网膜分布及中枢投射的证据。通过计算机辅助测量扁平视网膜和切片视网膜不同偏心度处的明场绘图来确定神经节细胞的大小。利用辣根过氧化物酶的逆行轴突运输研究了单个神经节细胞向背外侧膝状体核和上丘的投射模式。将过氧化物酶注入膝状体的小细胞层后，几乎每个神经节细胞都在视网膜的一个限定区域内被标记，该区域已知投射到紧邻注射针尖的膝状体区域。在注入大细胞层后，仅外周视网膜的最大细胞和中央凹旁约26%的神经节细胞被标记。将过氧化物酶注入上丘后，视网膜中各种大小的散在神经节细胞被标记，尽管在最中心的10度偏心度范围内未发现标记细胞。从这些观察结果得出结论，猕猴视网膜的所有神经节细胞都投射到背外侧膝状体的小细胞层，但只有外周视网膜的最大神经节细胞，而不是中央凹旁的所有细胞，投射到了大细胞层。相比之下，只有散在的神经节细胞，尽管大小各异，似乎投射到了上丘。文中描述了过氧化物酶示踪技术的两个主要问题：1. 注射部位周围可染色的过氧化物酶活性范围似乎大于实际可被轴突摄取以产生标记细胞的注射示踪剂面积。2. 切断或受损的轴突似乎能充分摄取过氧化物酶以产生细胞体的标记。
