Kaufmann S H, Blum C, Yamamoto S
Department of Immunology, University of Ulm, Federal Republic of Germany.
Proc Natl Acad Sci U S A. 1993 Oct 15;90(20):9620-4. doi: 10.1073/pnas.90.20.9620.
Although gamma/delta T cells express numerous in vitro functions similar to alpha/beta T cells, little is known about their biological functioning in vivo. Furthermore, it is unclear whether alpha/beta T cells and gamma/delta T cells act independently or in a coordinated way. In the present study, gamma/delta T cells were modulated in vivo by i.p. injection of the anti-gamma/delta T-cell receptor (TCR) monoclonal antibody GL3. GL3 administration caused disappearance of the gamma/delta TCR in spleen and lymph node cells and the gamma/delta TCR was reexpressed after in vitro cultivation for a few days. When cultured in vitro for 4 days, in the absence of foreign antigens, spleen and lymph node alpha/beta T cells from GL3-modulated mice showed vigorous proliferative responses. CD4 T lymphocytes from GL3-modulated mice produced interleukin 2, and CD8 T cells developed into cytolytic T lymphocytes in vitro capable of lysing syngeneic and allogeneic targets. Treatment with heat-inactivated GL3 or with normal hamster immunoglobulin did not cause any of these effects. These findings suggest that the anti-gamma/delta TCR monoclonal antibody GL3 modulates gamma/delta T cells in vivo and that this modulation has profound effects on alpha/beta T-cell reactivity. Hence, the data suggest a role for gamma/delta T cells in the regulation of alpha/beta T-cell activation in vivo.
尽管γ/δ T细胞在体外表现出许多与α/β T细胞相似的功能，但对于它们在体内的生物学功能却知之甚少。此外，尚不清楚α/β T细胞和γ/δ T细胞是独立发挥作用还是协同发挥作用。在本研究中，通过腹腔注射抗γ/δ T细胞受体（TCR）单克隆抗体GL3在体内对γ/δ T细胞进行调节。给予GL3导致脾脏和淋巴结细胞中的γ/δ TCR消失，并且在体外培养几天后γ/δ TCR重新表达。在无外源抗原的情况下于体外培养4天时，来自GL3调节小鼠的脾脏和淋巴结α/β T细胞表现出强烈的增殖反应。来自GL3调节小鼠的CD4 T淋巴细胞产生白细胞介素2，并且CD8 T细胞在体外发育成能够裂解同基因和异基因靶标的细胞毒性T淋巴细胞。用热灭活的GL3或正常仓鼠免疫球蛋白处理不会产生任何这些效应。这些发现表明，抗γ/δ TCR单克隆抗体GL3在体内调节γ/δ T细胞，并且这种调节对α/β T细胞反应性具有深远影响。因此，数据表明γ/δ T细胞在体内α/β T细胞活化的调节中起作用。
