Lipponen P K
Department of Pathology, University of Kuopio, Finland.
Pathobiology. 1993;61(3-4):178-82. doi: 10.1159/000163788.
The expression of c-erbB-2, p53 and proliferating cell nuclear antigen (PCNA) was studied using immunocytochemical techniques in paraffin-embedded biopsy specimens (consecutive 5-microns sections) from 104 transitional cell bladder tumours. 46/104 (44%) of the tumours were positive for c-erbB-2, 29/104 (28%) for p53 and 88/104 (85%) for PCNA. The expression of these antigens was independent of T-category, whereas the expression was significantly related to histological differentiation and papillary status so that non-papillary high-grade tumours were usually positive for all the antigens. The expression of c-erbB-2 and p53 was intense in 8/104 (8%) of cases and weak or absent in 76/104 (73%) of cases (p = 0.0003). The expression of p53 and PCNA was intense in 22/104 (21%) and weak or absent in 76/104 (73%) of tumours (p = 0.0003). Intense expression of c-erbB-2 and PCNA was also interrelated significantly (p = 0.0202). All three antigens were simultaneously expressed in 16-29% of T2-T4 tumours, in 22-35% of grade 2-3 tumours and in 35% of non-papillary tumours.
采用免疫细胞化学技术，对104例膀胱移行细胞肿瘤石蜡包埋活检标本（连续5微米切片）中c-erbB-2、p53和增殖细胞核抗原（PCNA）的表达进行了研究。104例肿瘤中46例（44%）c-erbB-2呈阳性，29例（28%）p53呈阳性，88例（85%）PCNA呈阳性。这些抗原的表达与T分期无关，而与组织学分化和乳头状状态显著相关，因此非乳头状高级别肿瘤通常所有抗原均呈阳性。104例病例中有8例（8%）c-erbB-2和p53表达强烈，76例（73%）表达较弱或无表达（p = 0.0003）。104例肿瘤中有22例（21%）p53和PCNA表达强烈，76例（73%）表达较弱或无表达（p = 0.0003）。c-erbB-2和PCNA的强烈表达也显著相关（p = 0.0202）。在T2-T4期肿瘤中，16%-29%的肿瘤同时表达这三种抗原；在2-3级肿瘤中，22%-35%的肿瘤同时表达这三种抗原；在非乳头状肿瘤中，35%的肿瘤同时表达这三种抗原。
