Sasaki Y, Honda M, Makino M, Sasaki T
Department of Safety Research on Biologics, National Institute of Health, Tokyo, Japan.
AIDS Res Hum Retroviruses. 1993 Aug;9(8):775-80. doi: 10.1089/aid.1993.9.775.
Because mycoplasma infection has often been observed in individuals infected with human immunodeficiency virus type 1 (HIV-1), we investigated the influence of mycoplasmas on HIV replication in vitro. Replication of HIV-1 assessed by reverse transcriptase activity and the p24 antigen level in peripheral blood mononuclear cells (PBMCs) was enhanced by the stimulation with the cell lysates of human mycoplasmas such as Mycoplasma pneumoniae, M. genitalium, and M. fermentans. The most drastic increase of HIV-1 replication was shown in M. pneumoniae, which induced an approximately 10-fold higher replication level than phytohemagglutinin. Stimulation with the mycoplasmas accompanied the induction of blastotransformation of CD4+ but not CD8+ T lymphocytes in PBMCs and the most predominant alterations were induced by the stimulation with M. pneumoniae among the species examined. Production of IL-2 was significantly increased by stimulation with all three species of Mycoplasma used. These results suggest that mycoplasmas induce vigorous replication of HIV-1 through the activation of CD4-positive T lymphocytes.
由于在感染1型人类免疫缺陷病毒（HIV-1）的个体中经常观察到支原体感染，我们在体外研究了支原体对HIV复制的影响。通过逆转录酶活性和外周血单核细胞（PBMC）中p24抗原水平评估的HIV-1复制，在受到人支原体（如肺炎支原体、生殖支原体和解脲脲原体）的细胞裂解物刺激后增强。HIV-1复制的最大幅度增加出现在肺炎支原体中，其诱导的复制水平比植物血凝素高约10倍。支原体刺激伴随着PBMC中CD4 +而非CD8 + T淋巴细胞的母细胞转化诱导，在所检测的物种中，肺炎支原体刺激诱导的变化最为显著。使用的所有三种支原体刺激均显著增加了IL-2的产生。这些结果表明，支原体通过激活CD4阳性T淋巴细胞诱导HIV-1的强烈复制。
